中国畜牧兽医2019，Vol.46Issue(1)：296-304,9.DOI:10.16431/j.cnki.1671-7236.2019.01.035
鹿角盘水提物对大鼠乳腺增生的作用效果及其机制研究
Effects of Deer Hard Antler Buttons Water Extracts on Hyperplasia of Mammary Glands in Rats and Underlying Mechanism
摘要
Abstract
To explore the therapeutic effects of deer hard antler button (DHAB) water extracts on hyperplasia of mammary glands (HMG) in rats and to explore its mechanism of action and dosage, the model of HMG was established through intramuscular injection of estradiol benzoate (0.5mg/ (kg·d) ) and progesterone (5.0mg/ (kg·d) ) in 70 SD rats.There were 7groups with10 rats in each group, which were divided into blank group, HMG model group, positive drug tamoxifen group (0.036mg/ (kg·d) ) , 4DHAB groups (high-dose, H, 2.625g/ (kg·d) ;Medium-high-dose, MH, 1.575g/ (kg·d) ;Medium-low-dose, ML, 1.05g/ (kg·d) ;Low-dose, L, 0.63g/ (kg·d) ) .Each group was treated with intragastric administration for 45 d.The diameter and height of nipple and body weight were recorded weekly.Blood samples were taken and thymus, spleen, uterus and ovary weights of the rat breasts were measured at the end of the test.Histopathological sections and HE staining were performed and sex hormone estradiol (E2) and progesterone (P) were detected by radioimmunoassay.The results indicated that different doses of DHAB water extracts could reduce the diameter and height of nipple, increase thymus and spleen indexes and reduce uterine index, reduce the number of breast lobules, acinar numbers and secretion contents, and decrease E2 and increase P levels in serum of HMG rats.All these indexes were significantly or extremely significantly different from the model group (P<0.01;P<0.05) .In conclusion, DHAB water extracts had significant therapeutic effects on HMG in rats, and the medium-high-dose was the best.The action mechanism of DHAB water extracts was enhancing immunity and regulating serum E2 and P levels.关键词
鹿角盘水提物/乳腺增生症 (HMG)/治疗效果/作用机制Key words
deer hard antler button (DHAB) water extracts/hyperplasia of mammary glands (HMG) /therapeutic effect/mechanism of action分类
农业科技引用本文复制引用
关晴,赵海平,王东旭,王玉俗,李春义,徐莉..鹿角盘水提物对大鼠乳腺增生的作用效果及其机制研究[J].中国畜牧兽医,2019,46(1):296-304,9.基金项目
吉林省重点自然科学基金(20170101003JC) （20170101003JC）
中央级公益性科研院所基本科研业务费专项(1610342016021) （1610342016021）
