中国畜牧兽医2019，Vol.46Issue(1)：305-312,8.DOI:10.16431/j.cnki.1671-7236.2019.01.036
复方诃子口服液抗犊牛大肠杆菌性腹泻的药理作用研究
Studies on the Pharmacological Effects of Compound Myrobalan Oral Liquid Against Escherichia coli Diarrhea in Calves
摘要
Abstract
The aim of the experiment was to study the pharmacological effects of compound Myrobalanoral liquid against Escherichia coli (E.coli) diarrhea in calves.We used neutralize enterotoxin test, anti-exudation test, antipyretic test, analgesic test, intestinal peristalsis test and immunomodulation test to verify the pharmacological effects of compound Myrobalan oral liquid.Theresults showed that in the neutralize enterotoxin test, there was extremely significant difference between compound Myrobalanoral liquid and negative control groups (P<0.01) .In the anti-exudation test, all drug groups could reduce the exudation of Evans blue to varying degrees, the inhibition rates of the aspirin group, compound Myrobalan oral liquid high, medium and low dose groups were 58.1％, 42.8％, 31.6％ and 14.9％, respectively.Among them, the compound Myrobalanoral liquid low dose group was significantly different from blank group (P<0.05) , while other groups were extremely significantly different from blank group (P<0.01) .The compound Myrobalan oral liquid medium and low dose groups had extremely significant difference compared with aspirin group (P<0.01) , but the compound Myrobalan oral liquid high dose group had no significant difference (P>0.05) .In the antipyretic test, the high-dose group of compound Myrobalan oral liquid quickly inhibited the increase of body temperature 1hafter administration, and the increase of body temperature was inhibited 2hafter the administration in medium dose group;3h after the administration, the body temperature was significantly reduced in high and medium dose groups compared with model group;The difference between low dose and model groups was not significant.In the anti-analgesic test, there were extremely significant differences in aspirin group, compound Myrobalanoral liquid high and medium dose groups compared with blank group (P<0.01) , the writhing inhibition rates were 64.55％, 52.73％and 32.73％, respectively, the pain latency were 9.59, 6.33 and 5.74min;And there was no significant difference in low dose group (P>0.05) .In the intestinal peristalsis test, compared with blank group, compound Myrobalan oral liquid high dose and atropine sulphate groups had obviously inhibitory effect on intestinal peristalsis (P<0.01) , and the promotion rates were 44.18％ and 52.55％, respectively;The effect of medium dose group of compound Myrobalan oral liquid was significant different (P<0.05) , low dose group of compound Myrobalanoral liquid was not significant (P>0.05) .In the immunomodulation test, the white blood cell (WBC) count, spleen and thymus organ indexes in model group were significantly lower than those in blank group (P<0.05) , and the WBC count, spleen and thymus organ indexes in compound Myrobalan oral liquid group were significantly higher than those in model group on the 14 th day (P<0.05) .In conclusion, compound Myrobalan oral liquid had neutralize enterotoxin, anti-exudation of tissue fluid, antipyretic, analgesia and inhibitory effect on intestinal peristalsis of E.coli diarrhea, and could enhance the immune function and other pharmacological effects.关键词
复方诃子口服液/大肠杆菌/腹泻/药理作用Key words
compound Myrobalanoral liquid/Escherichia coli/diarrhea/pharmacological effects分类
农业科技引用本文复制引用
韩玉文,李子佳,于文会,王娜,姜晓文,郝敬友..复方诃子口服液抗犊牛大肠杆菌性腹泻的药理作用研究[J].中国畜牧兽医,2019,46(1):305-312,8.基金项目
公益性行业(农业)科研专项(201403051) （农业）
