中国医科大学学报2019，Vol.48Issue(1)：71-74,4.DOI:10.12007/j.issn.0258-4646.2019.01.014
miR-24对肺癌生物学功能的抑制作用
miR-24 Inhibits Lung Cancer Cell Proliferation and Migration
王黎明 1杨大业 1仇剑 1张德巍1
作者信息
- 1. 中国医科大学附属第四医院第三普通外科, 沈阳 110032
- 折叠
摘要
Abstract
Objective To investigate how microRNA 24 (miR-24) regulates the development of lung cancer. Methods The levels of miR-24 in 30 cases of lung cancer were detected using real-time PCR, and the relationship between miR-24 levels and overall survival was analyzed. After overexpression or silencing of miR-24 in A549 lung cancer cells, the effect on cell proliferation was observed by the MTT assay. Transwell assays were carried out to observe the effect of miR-24 on cell migration. The effects of miR-24 on the expression of cyclin-dependent kinase (CDK) 4/6 and matrix metalloproteinase (MMP) 2 in A549 cells were examined by Western blotting. Results Survival analysis showed that patients with low miR-24 expression had a shorter survival time. The MTT-based viability assay revealed that overexpression of miR-24 inhibited A549 cell proliferation. Cell proliferation was promoted when miR-24 was inhibited. In the Transwell assay, overexpression of miR-24 significantly inhibited the A549 cell migration, and cell migration increased when miR-24 was inhibited.Western blotting analysis revealed that overexpression of miR-24 could inhibit CDK4/6 and MMP2 production in A549 cells. Inhibition of miR-24 was associated with increased production of CDK4/6 and MMP2 in A549 cells. Conclusion miR-24 can inhibit the proliferation and migration of A549 lung cancer cells.关键词
miR-24/肺癌/A549/增殖/迁移Key words
miR-24/lung cancer/A549/proliferation/migration分类
医药卫生引用本文复制引用
王黎明,杨大业,仇剑,张德巍..miR-24对肺癌生物学功能的抑制作用[J].中国医科大学学报,2019,48(1):71-74,4.
