中国医科大学学报2019，Vol.48Issue(2)：109-113,5.DOI:10.12007/j.issn.0258-4646.2019.02.004
TLR4/Myd88/NF-κB通路介导表没食子儿茶素没食子酸酯对脓毒血症大鼠急性肾损伤的保护作用
Protective Effect of Epigallocatechin Gallate on Acute Kidney Injury Induced by Lipopolysaccharide in Rats via TLR4/Myd88/NF-κB Pathway
摘要
Abstract
Objective To evaluate the protective effect of epigallocatechin gallate (EGCG) on lipopolysaccharide (LPS) -induced acute kidney injury (AKI) in rats and its underlying mechanisms. Methods Sprague-Dawley rats were randomly divided into the Sham group, AKI group, EGCG group and TLR4 group (n = 10 each). To establish the rat model of endotoxemia, serum creatinine (Cr) and urea nitrogen (BUN) levels were detected by biochemical assays; serum interlukin (IL) -6, IL-1β, IL-10, and TNF-α levels were detected by ELISA; kidney histopathology was examined by hematoxylin and eosin (HE) staining method; and expression of TLR4, Myd88 and nuclear factor-kappa B (NF-κB) in rat kidneys at both protein and mRNA levels was detected by Western blotting and qRT-PCR, respectively.Results Kidney injury increased significantly in AKI group compared to the sham group. Serum Cr, BUN, IL-6, IL-1β, and TNF-α levels significantly increased whereas IL-10 levels significantly decreased in AKI group compared to the sham group. Expression levels of TLR4, Myd88, and NF-κB also significantly increased at both protein and mRNA levels in AKI group compared to the sham group. Treatment with EGCG prior to induction of LPS-mediated AKI conferred protection against AKI by significantly reducing the expression of inflammatory markers such as, TLR4, Myd88, and NF-κB. Given TLR4 inhibitor based on this, the protective effect of EGCG on AKI was via inhibition of the TLR4/Myd88/NF-κB pathway. Conclusion EGCG exhibited a protective effect against LPS-induced AKI by inhibiting the activation of TLR4/Myd88/NF-κB pathway.关键词
表没食子儿茶素没食子酸酯/急性肾损伤/TLR4/核因子kappa B/脂多糖Key words
pigallocatechin gallate/acute kidney injury/TLR4/nuclear factor-kappa B/lipopolysaccharide分类
医药卫生引用本文复制引用
李木子,陈克研,孙倩,邱雨华..TLR4/Myd88/NF-κB通路介导表没食子儿茶素没食子酸酯对脓毒血症大鼠急性肾损伤的保护作用[J].中国医科大学学报,2019,48(2):109-113,5.基金项目
国家自然科学基金青年科学基金 (31201758) （31201758）
