中国医科大学学报2019，Vol.48Issue(2)：114-118,5.DOI:10.12007/j.issn.0258-4646.2019.02.005
脂肪干细胞来源外泌体通过Wnt/β-catenin信号通路促进脂肪干细胞成骨分化
Exosomes Promote Osteoblastic Differentiation of Adipose-Derived Stem Cells by Activating the Wnt/β-catenin Signaling Pathway
摘要
Abstract
Objective To investigate the relationship between promotion of osteogenic differentiation of adipose-derived stem cells (ADSCs) by exosomes secreted by ADSCs and the Wnt/β-catenin signaling pathway. Methods ADSCs were extracted in the Plastic Surgery Department of the First Hospital Affiliated to China Medical University. Exosomes were extracted from ADSCs induced to differentiate into osteoblasts. The ADSCs were separated into four groups and used for measuring activity of alkaline phosphatase (ALP) by using enzyme-labeled assay, formation of calcium nodes by Alizarin red staining, and expression of β-catenin by Western blotting. Results ADSC-derived exosomes promoted differentiation of ADSCs into osteoblasts by upregulating the expression of β-catenin. Addition of Dickkopf-related protein 1 (DKK1), an inhibitor of Wnt/β-catenin pathway, inhibited expression of β-catenin which in turn resulted in reduced potential of ADSCs for osteogenic differentiation. Conclusion The osteogenic differentiation of ADSCs can be promoted by exosomes derived from ADSCs via the Wnt/β-catenin signaling pathway.关键词
脂肪干细胞/外泌体/Wnt/β-catenin信号通路/成骨分化Key words
adipose-derived stem cells/exosomes/Wnt/β-catenin signaling pathway/osteogenic differentiation分类
医药卫生引用本文复制引用
崔梦莹,郭澍,佟爽,张华,朱梦茹,陈义庆,魏松乔..脂肪干细胞来源外泌体通过Wnt/β-catenin信号通路促进脂肪干细胞成骨分化[J].中国医科大学学报,2019,48(2):114-118,5.基金项目
国家自然科学基金 (51272286) （51272286）
国家卫生和计划生育委员会公益性科研专项 (2015SQ00049) （2015SQ00049）
辽宁省自然科学基金 (20170541040) （20170541040）
