中国医科大学学报2019，Vol.48Issue(2)：124-127,4.DOI:10.12007/j.issn.0258-4646.2019.02.007
跨膜蛋白16F对乳腺癌细胞增殖及迁移的影响
Effects of TMEM16F on the Proliferation and Migration of Breast Cancer Cells
摘要
Abstract
Objective To investigate the expression of transmembrane protein 16 F (TMEM16 F) in breast cancer cells and to elucidate the effect of the knockdown of its gene on proliferation and migration of T47 D breast cancer cells. Methods The expression of TMEM16 F protein in MDA-MB-231 and T47 D breast cancer cells was detected by Western blotting. The effect of TMEM16 F knockdown on the proliferation of T47 D breast cancer cells was detected by CCK-8 cell proliferation assay, and its effect on the migration of T47 D breast cancer cells was examined by cell scratch assay. Results Western blotting results showed TMEM16 F protein expression in breast cancer cells.The results of CCK-8 cell proliferation assay showed that compared to the Scrambled shRNA group, the proliferation of T47 D breast cancer cells was decreased after TMEM16 F knockdown (P = 0.037 0). The results of the scratch test showed that the migration ability of T47 D breast cancer cells was enhanced after TMEM16 F knockdown, compared to the control group (P = 0.002 7). Conclusion TMEM16 F is highly expressed in T47 D breast cancer cells. Knockdown of TMEM16 F can inhibit the proliferation and promote the migration of T47 D breast cancer cells.关键词
乳腺癌/跨膜蛋白16F/增殖/迁移Key words
breast cancer/transmembrane protein 16F/proliferation/migration分类
医药卫生引用本文复制引用
樊璐,王慧,肖庆桓..跨膜蛋白16F对乳腺癌细胞增殖及迁移的影响[J].中国医科大学学报,2019,48(2):124-127,4.基金项目
国家自然科学基金 (81572613, 81702611) （81572613, 81702611）
