中国油料作物学报2019，Vol.41Issue(1)：25-32,8.DOI:10.7505/j.issn.1007-9084.2019.01.004
小桐子ACO1基因的克隆与表达分析
Cloning and expression analysis of ACO1 Gene in Jatropha curcas
摘要
Abstract
In order to study the role of JcACO1 gene in stress resistance of Jatropha curcas, the full-length coding frame sequence of ACO1 (1-aminocyclopropane-1-carboxylic acid oxidase) gene, named JcACO1, was cloned based on the genome database of J. curcas, and its functional domain, phylogenetic evolution, gene structure and low-temperature expression characteristics were analyzed. The results showed that the length of the cloned JcACO1 gene was 1 022 bp, encoded 319 amino acids with the molecular weight of 36. 04 kDa and the pI value of5. 50. Sequence alignment demonstrated that JcACO1 was highly conserved in the middle of the sequence and had a conserved domain of Pcbc superfamily. Phylogenetic tree analysis showed that the JcACO1 had high homology with Populus trichocarpa, Hevea brasiliensis and Manihot esculenta. qRT-PCR analysis revealed that Jatropha curcas seeding JcACO1 expressed in different organs, with abundant expression in leaves and stems, but scarcely in roots. In addition, JcACO1 gene was induced by hypothermia in all three organs, and the highest expression of JcACO1 gene was observed in roots and stems at 24 h of low temperature stress. The results showed that the expression of JcACO1 gene was increased in response to chilling stress.关键词
小桐子/ACO1/基因克隆/表达分析Key words
Jatropha curcas/ACO1/gene cloning/expression analysis分类
生物科学引用本文复制引用
辛胡,唐利洲,付义,蒋凯文,杨蕾,刘家奇,马楠,田斌,王海波..小桐子ACO1基因的克隆与表达分析[J].中国油料作物学报,2019,41(1):25-32,8.基金项目
国家自然科学基金 (31460179) （31460179）
