中国油料作物学报2019，Vol.41Issue(1)：109-114,6.DOI:10.7505/j.issn.1007-9084.2019.01.014
花生荚壳抗黄曲霉菌侵染的鉴定方法研究及抗性种质发掘
Method for screening resistance of peanut shell to Aspergillus flavus infection and identification of resistant genotypes
摘要
Abstract
Aflatoxin contamination has been a key factor affecting food safety of peanut products and a serious constraint to peanut industry. The resistance to infection of Aspergillus flavus in shell is the preliminary physical protection mechanism for reducing risk of toxin contamination in peanut. However, very limited research has been done on identification method for shell resistance to A. flavus infection. In this research, peanut pods were inoculated with A. flavus strain AF2202 with 5 concentrations of inoculum solutions and then incubated for 3-9 days in darkness. Through comparing various combinations of inoculation and incubation period, the optimal combination for identifying shell resistance was 2 × 106 spores per milliliter and 7 days. A total of 276 diversified lines consisting of peanut core collections were tested using the newly-developed method, and the results further confirmed that the method was effective and practicable. Significant difference in shell resistance was found among the involved germplasm lines and 2 elite lines with shell resistance were identified.关键词
花生荚壳/黄曲霉菌/侵染抗性Key words
peanut shell/Aspergillus flavus/infection resistance分类
农业科技引用本文复制引用
邱西克,雷永,廖伯寿,康彦平,郭建斌,喻博伦,陈伟刚,姜慧芳,黄莉,李威涛,罗怀勇..花生荚壳抗黄曲霉菌侵染的鉴定方法研究及抗性种质发掘[J].中国油料作物学报,2019,41(1):109-114,6.基金项目
国家自然科学基金 (31461143022) （31461143022）
国家花生产业技术体系 (CARS-13) （CARS-13）
农作物种质资源保护项目 (2018NWB033) （2018NWB033）
国家野生花生种质资源共享平台 (NICGR2018-36) （NICGR2018-36）
