中国油料作物学报2019，Vol.41Issue(1)：115-119,5.DOI:10.7505/j.issn.1007-9084.2019.01.015
花生疮痂病菌毒素的致病作用及其生理生化研究
Pathogenesis and physiological and biochemical responses of toxin produced by Elsino(e) arachidis
摘要
Abstract
Elsinochrome (ESC) produced by Elsino? arachidis (Bitauc. & Jenkins) Rossman & W. C. Allen is a photosensitizing perylenequinone toxins, which performed crucial functions in the process of pathogen infection.In order to investigated a mechanism by which Elsinochrome contributes to the pathogenic success of this fungus, the content of reactive oxygen species, the activity of reactive oxygen scavenging enzymes and the degree of lipid membrane peroxidation in the host were systematically studied. The results showed that, after toxin inoculation, the content of superoxide anions (O2-) and hydrogen peroxide (H2 O2) in the leaves peaked at 8 h and reached at1 482. 99 U·L-1 and 25. 25μmol·g-1, respectively. It was 10 and 40 times as those of the clear water control.The activity peaks of SOD, CAT and POD in the active oxygen scavenging system appeared at 48 hours after inoculation, which were 78. 00 U·mg-1, 16. 20 U·mg-1 and 63. 73 U·mg-1, respectively, and then decreased to the control level after 96 h. Moreover, MDA content peaked after 72 h, the peak value was 5. 44μmol·g-1, and the membrane permeability increased with the inoculation time, up to 80％ at 96 h. The results suggested that the toxin induced necrotic lesions on peanut leaves, promoted the level of lipid peroxidation and killed cells by producing ROS. Thus, the toxin produced by Elsino? arachidis was directly toxic in peanut.关键词
花生疮痂病/痂囊腔菌素/真菌毒素/活性氧/质膜过氧化Key words
peanut scab/elsinochrome/mycotoxin/reactive oxygen species/lipid peroxidation分类
农业科技引用本文复制引用
焦文莉,刘璐,周如军,许梦雪,肖迪,傅俊范..花生疮痂病菌毒素的致病作用及其生理生化研究[J].中国油料作物学报,2019,41(1):115-119,5.基金项目
辽宁省农业领域青年科技创新人才培养计划项目 (2014056) （2014056）
