中国油料作物学报2019，Vol.41Issue(1)：130-143,14.DOI:10.7505/j.issn.1007-9084.2019.01.018
响应面法优化磷酸化改性花生分离蛋白-多肽膜的制备工艺
Optimization of phosphorelated peanut protein isolate-polypeptide films preparation by response surface methodology
摘要
Abstract
In order to optimize the preparation process conditions of phosphorelated peanut protein isolate-polypeptide films, experiment design was carried out with the response surface Box-Benhnken experiment, on the basis of single factor experiments. The results showed that the optimum conditions obtained were protein concentration, pH value, glycerol percentage content of protein, xanthan gum percentage content of protein, time, temperature, ultrasonic power, ultrasonic frequency, polypeptide concentration of 8％, 8. 2, 13. 4％, 1％, 60 min, 69℃, 270 W, 28 kHz, 61 mg/mL, respectively. Under the optimum conditions, the theoretical predictive values of film thickness, water absorption and light transmittance were 86μm, 41. 9％ and 53. 6％, respectively. The results of verification experiment showed that film thickness, water absorption rate and light transmittance rate could reach88 ± 2μm, 43. 1 ± 1. 2％ and 52. 4 ± 1. 5％, respectively. The differences between experimental and theoretical values were 2. 33％, 2. 86％ and 2. 24％, respectively. This suggested the response surface quadratic model fitted well. The functional properties were better than those of phosphorelated modified peanut protein isolate films, and these functional properties included tensile strength of 9. 62 MPa, elongation of 101. 68 ％, solubility of 47. 69 ％, water vapor permeability of 6. 95 g·m-2·h-1 and antioxidant activities including DPPH free radical scavenging activity of IC507. 70 mg·mL-1, hydroxy free radical scavenging activity of IC505. 98 mg·mL-1, superoxide anion free radical scavenging activity of IC504. 20 mg·mL-1, iron ion chelating activity of IC503. 79 mg·mL-1, copper ion chelating activity of IC5013. 61 mg·mL-1, lipid peroxidation inhibition activity of IC508. 62 mg·mL-1, iron reducing power of IC5013. 93 mg·mL1, molybdenum reducing power of IC505. 49 mg·mL-1. The results of this study provided a new way for the application of phosphorelated modified peanut protein isolate.关键词
磷酸化改性花生分离蛋白-多肽膜/制备工艺/响应面法/抗氧化活性Key words
phosphorelated modified peanut protein isolate-polypeptide films/preparation process/response surface methodology/antioxidant activities分类
轻工纺织引用本文复制引用
于丽娜,王明清,杜德红,彭娅萍,杨伟强,李勇,顾博,孙杰,张初署,毕洁..响应面法优化磷酸化改性花生分离蛋白-多肽膜的制备工艺[J].中国油料作物学报,2019,41(1):130-143,14.基金项目
山东省自然科学基金 (ZR2016CM43, ZR2016YL021, ZR2017MC060, ZR2017MC062) （ZR2016CM43, ZR2016YL021, ZR2017MC060, ZR2017MC062）
山东省重点研发计划 (2015GGX108006) （2015GGX108006）
山东省农业科学院重大科技成果培育计划 (2016CGPY10) （2016CGPY10）
山东省农业科学院青年科研基金 (2016YQN18) （2016YQN18）
