中国药物经济学2019，Vol.14Issue(1)：30-34,5.DOI:10.12010/j.issn.1673-5846.2019.01.006
基于代谢组学的生脉注射液治疗脓毒症作用机制研究
Research on Mechanism of Shengmai Injection (生脉注射液) in the Treatment of Sepsis Based on Metabolomics
摘要
Abstract
Objective To explore the mechanism of Shengmai Injection (生脉注射液) in treating sepsis with multi-component, multi-target and multi-pathway by metabolomics. Methods The sepsis animal model was established by the internationally recognized CLP method of cecum ligation and perforation. Rats in blank group and model group were intraperitoneally injected with 0.9％ saline 0.2 mL and rats in Shengmai Injection group were injected with Shengmai Injection 0.2 mL (2 mg/kg·min). On this basis, metabolomics study of rat urine was carried out. Through full spectrum analysis of high resolution mass spectrometry data, biological information of terminal metabolites in septic rats was obtained, and key metabolic enzymes or metabolic pathways were focused to clarify the intervention effect of Shengmai Injection on sepsis. Results The urine metabolic profile showed that the metabolic distribution of rats in blank group and model group was distinctly separated. The Shengmai Injection group was between the two groups and was close to the blank group, which proved that Shengmai Injection had a good therapeutic effect on septic rats. Further, the metabolites were identified by focusing on the small molecule metabolites with abnormal metabolic distribution and comparing with the secondary metabolites in the human metabolome database, namely proline, L-methionine, leucine, arginine and taurine. The pathway enrichment analysis showed that the metabolism of sepsis rats mainly involved in taurine and taurine metabolism, arginine and proline metabolism, β-alanine metabolism, glutathione metabolism, cysteine and methionine metabolism. Conclusion Shengmai Injection has significant therapeutic effect on septic rats. Regulation of taurine and taurine metabolism, arginine and proline metabolism may be the key mechanism in the treatment of sepsis.关键词
生脉注射液/脓毒症/代谢组学/代谢通路/生物标记物Key words
Shengmai Injection (生脉注射液)/Sepsis/Metabolomics/Metabolic pathways/Biomarkers分类
医药卫生引用本文复制引用
柴瑞平,张晏航,路娟,王同瑾,陈曦..基于代谢组学的生脉注射液治疗脓毒症作用机制研究[J].中国药物经济学,2019,14(1):30-34,5.基金项目
国家自然科学基金面上项目(81673667) （81673667）
