中国药物评价2018，Vol.35Issue(6)：428-431,4.
HPLC-PDA法同时测定金嗓子喉片中没食子酸和绿原酸的含量
Simultaneous Determination of Gallic Acid and Chlorogenic Acid in Golden Throat Lozenge by HPLC-PDA
刘静 1董宇 1母芹 2王震红1
作者信息
- 1. 辽宁省药品检验检测院,辽宁 沈阳 110036
- 2. 沈阳药科大学,辽宁 沈阳 110016
- 折叠
摘要
Abstract
Objective:High performance liquid chromatography-photodiode array (HPLC-PDA) method was developed for the determination of gallic acid and chlorogenic acid in Golden Throat Lozenge.Methods:The separation of gallic acid and chlorogenic acid was performed on a SHISEIDO HPLC PACKED C18column (4.6 mm×250 mm, 5μm) with the mobile phase consisting of acetonitrile-0.1％phosphoric acid in a gradient elution mode at a flow rate of 1.0 m L·min-1.Ultraviolet absorption detector was set at 270 nm for gallic acid and 330 nm for chlorogenic acid.The column temperature was set at 25℃.Results:Good linearity was obtained in the ranges of10.02-80.16μg·m L-1 (r=0.999 8) for gallic acid and 5.973-47.78μg·m L-1 (r=0.999 7) for chlorogenic acid, respectively.The spiked recoveries were 100.3％and 99.6％with the relative standard derivations (RSDs) of 0.59％and 0.85％for gallic acid and chlorogenic acid, respectively.The limits of quantification (LOQs) (S/N=10) were 0.1μg·m L-1and 0.06μg·m L-1, and the limits of detection (LODs) (S/N=3) were 0.06μg·m L-1and 0.01μg·m L-1for gallic acid and chlorogenic acid, respectively.Conclusion:The results demonstrated that the proposal method is simple, accurate, reproducible and suitable for quality control of Golden Throat Lozenge.关键词
金嗓子喉片/没食子酸/绿原酸/二极管阵列检测器色谱 (HPLC-PDA) 法Key words
Golden Throat Lozenge/Gallic acid/Chlorogenic acid/High performance liquid chromatography-photodiode array (HPLC-PDA) 分类
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刘静,董宇,母芹,王震红..HPLC-PDA法同时测定金嗓子喉片中没食子酸和绿原酸的含量[J].中国药物评价,2018,35(6):428-431,4.
