中国医学创新2019，Vol.16Issue(1)：27-31,5.DOI:10.3969/j.issn.1674-4985.2019.01.007
雌二醇通过上调M-CSF表达促进乳腺癌教化单核细胞
Estradiol May Upregulate M-CSF to Promote Breast Cancer Recruit and Activate Monocytes
摘要
Abstract
Objective:To investigate the effect of Estradiol on educating monocyte differentiation into tumor associated macrophage in MCF-7 coculture system.Method:48 h after treated with 10 nM Estradiol or10 nM Estradiol+1μM Tamoxifen, MCP-1 and M-CSF in human breast cancer cell line MCF-7 culture supernatant were detected, they were given Transwell niche to further explore their effects on the recruitment and activation of monocyte.Result:Bio-Rad cytokine chip assay showed that the M-CSF concentration in the supernatant of blank control group, Estradiol group and Estradiol+Tamoxifen group were (1.83±0.18), (29.71±8.06) and (10.90±2.38) pg/mL, and the MCP-1 concentration were (31.33±2.40), (75.37±5.50) and (41.97±5.80) pg/mL respectively, the differences were statistically significant (P<0.05).The level of CD163 mRNA in estradiol treatment group was (76.83±12.80) times higher than that in control group, and that in Estradiol+Tamoxifen group was (6.50±1.71) times higher than that in control group (F=96, P<0.01).The number of U937 cells in the control group, the Estradiol treatment group and the Estradiol+Tamoxifen treatment group were (28±6), (188±16) and (82±9) respectively, the difference was statistically significant (F=160, P<0.05).Conclusion:Estradiol may promote hormone dependent breast cancer cell lines MCF-7 to secrete MCP-1 and M-CSF, thereby educate monocyte differentiation into tumor associated macrophage, while Tamoxifen may partially block the effect.关键词
激素依赖型乳腺癌/雌二醇/他莫昔芬/MCP-1/M-CSFKey words
Hormone dependent breast cancer/Estradiol/Tamoxifen/MCP-1/M-CSF引用本文复制引用
季秋兰,丁景弦..雌二醇通过上调M-CSF表达促进乳腺癌教化单核细胞[J].中国医学创新,2019,16(1):27-31,5.基金项目
江西省自然科学基金(20171BAB205057) （20171BAB205057）
