中国医学创新2019，Vol.16Issue(1)：31-35,5.DOI:10.3969/j.issn.1674-4985.2019.01.008
溃疡性结肠炎大鼠肠黏膜中E选择素与P选择素的表达及意义
Expression and Significance of E-selectin and P-selectin in Intestinal Mucosa of Rats with Ulcerative Colitis
摘要
Abstract
Objective:To investigate the expression levels and significance of E-selectin and P-selectin in intestinal mucosa of rats with ulcerative colitis (UC).Method:A total of 65 healthy male (Sprague Dawkey, SD) rats were selected from March to June 2018, they were divided into blank control group (n=12) and experimental group (n=53).The blank control group was not treated, 12 SD rats were randomly selected from the experimental group for the pre-experiment, and the most appropriate dose of TNBS (2, 4, 6-trinitrobenzene sulfonic acid) was screened to build the model of ulcerative colitis.The remaining rats were formally moulded and divided into group A, group B and group C according to different anatomical time, with 12 rats in each group.Through daily observation of body weight and changes in general conditions (mental state, shape and color of feces, etc.), according to disease activity index (DAI) score, the status of experimental rats were recorded and the severity of ulcerative colitis in rats was preliminarily determined.Rats were sacrificed on 3, 7 and 14 d respectively, and colon macroscopic damage index (CMDI) was used to assess the degree of ulceration.ELISA method was used to detect the content indexes of E-selectin and P-selectin in intestinal mucosa, and meanwhile the pathological changes of Colon were evaluated.The correlation between the content of E-selectin and P-selectin and the degree of ulcer was obtained.Result:The DAI and CMDI scores of rats in the experimental group were significantly higher than those in the blank control group, and the pathological changes of colonic ulcer in the experimental group were significantly changed under the microscope.The contents of E-selectin and P-selectin in blank control group, A, B and C groups were compared, the differences were statistically significant (P<0.05).In the course of recovery from ulcerative colitis, the levels of E-selectin and P-selectin in intestinal mucosa of experimental group increased gradually at first and then decreased, but the contents of E-selectin and P-selectin in group C were still significantly higher than those in group A.Conclusion:During the development of ulcerative colitis, the levels of E-selectin and P-selectin increased with the aggravation of the degree of colon ulcer.The levels of E selectin and P-selectin decreased with the decrease of degree of colon ulcer.E-selectin and P-selectin may play a role in promoting the development of UC, which can be used as a dynamic reference indicator for the diagnosis of UC and provide a new direction for the clinical study on the pathogenesis of UC.关键词
UC/SD大鼠/CMDI/DAI/P选择素/E选择素Key words
UC/SD rats/CDMI/DAI/P-selectin/E-selectin引用本文复制引用
李威,李震,陈君卓,赵君雅,徐杰,于鹏,官杰..溃疡性结肠炎大鼠肠黏膜中E选择素与P选择素的表达及意义[J].中国医学创新,2019,16(1):31-35,5.基金项目
2018年黑龙江省大学生创新创业项目(201811230012) （201811230012）
2017年黑龙江省大学生创新创业项目(20171123002) （20171123002）
2017年齐齐哈尔医学院团委课外科研课题(第15批) （第15批）
