中国医学创新2019，Vol.16Issue(3)：6-10,5.DOI:10.3969/j.issn.1674-4985.2019.03.002
替莫唑胺联合氟桂利嗪对胶质瘤细胞体外作用机制
Mechanism of Temozolomide Combined with Flunarizine in Glioma Cell Lines in Vitro
卢家潮 1寿记新 1李龙龙 1王冰冰1
作者信息
- 1. 郑州大学第五附属医院,河南 郑州 450052
- 折叠
摘要
Abstract
Objective:To investigate the effect of Temozolomide (TMZ) combined with Flunarizine (FZ) on glioma cell lines in vitro.Method:The glioma U87, U251, CHG-5 and SHG-44 cell lines were cultured in Eagle's medium, Cell Counting Kit-8 (CCK-8) assay was used to evaluate the cell proliferation in glioma cell lines which treated with TMZ combined with FZ either alone or in combination.Result:FZ inhibited the proliferation of glioma cell lines, but it was far less therapeutic effect.In the same kind of cells, the 50％ inhibitory concentration (IC50) of FZ in cell lines U87, U251, CHG-5, SHG-44 were (562.3±56.7), (370.5±40.2), (480.9±29.6), (420.5±61.4) μg/mL.The IC50 of TMZ was significantly decreased after different concentrations of FZ combined with TMZ (P＜0.05).Compared with the control group, TMZ and FZ monotherapy group, the apoptotic rate of TMZ combined with FZ induced a significant increase of glioma cells.Conclusion:FZ could inhibit the proliferation of glioma U87, U251, CHG-5 and SHG-4, and it could improve the chemotherapy effect when combined with TMZ.关键词
替莫唑胺/氟桂利嗪/胶质瘤Key words
Temozolomide/Flunarizine/Glioma引用本文复制引用
卢家潮,寿记新,李龙龙,王冰冰..替莫唑胺联合氟桂利嗪对胶质瘤细胞体外作用机制[J].中国医学创新,2019,16(3):6-10,5.
