中国医学创新2019，Vol.16Issue(3)：29-32,4.DOI:10.3969/j.issn.1674-4985.2019.03.007
四种常见离体脱位牙贮存介质对其牙周膜成纤维细胞生物活性的影响
Effect of Four Common in Vitro Dislocated Teeth Storage Medium on the Biological Activity of Periodontal Ligament Fibroblasts
摘要
Abstract
Objective:To evaluate the effect of four common in vitro dislocated teeth storage medium on the biological activity of periodontal ligament fibroblasts.Method:A total of 200 mandibular first premolars extracted from 200 cases of 14-22 year old adolescents in our hospital due to orthodontic treatment from January 2017 to January 2018 were selected as the research objects.They were randomly divided into four experimental groups (saline group, milk group, saliva group, tap water group) and control group, 40 teeth in each group.The periodontal ligament fibroblasts were cultured in the dislocation teeth.The experimental group were treated with saline, milk, saliva and tap water as storage medium respectively, while the control group was stored directly in air.The periodontal ligament cells were detected by CCK-8 method in the storage medium of four common in vitro dislocation teeth for 2, 6, 12 and 24 h respectively.The activity at different time points were compared with the control group.Result:The survival rate of periodontal ligament cells in each experimental group at different time points were significantly higher than those in the control group, the differences were statistically significant (P＜0.05).The survival rate of periodontal ligament cells in the saline, milk and saliva groups at different time points were significantly higher than those in the tap water group, the differences were statistically significant (P＜0.05).The survival rate of periodontal ligament cells in the milk and saliva groups at different time points were significantly higher than those in the saline group, and the survival rate of periodontal ligament cells in the milk group at 12 and 24 h was significantly higher than that in the saliva group, the differences were statistically significant (P＜0.05).Conclusion:The periodontal ligament fibroblasts stored in milk and saliva have higher activity and can be used as effective temporary storage solution for dislocated teeth in vitro.The storage effect of saline is the second, while that of tap water is the worst, and it is not suitable for storage medium.关键词
牙周膜成纤维细胞/脱位牙/贮存介质/生物学活性Key words
Periodontal ligament fibroblasts/Dislocated teeth/Storage medium/Biological activity引用本文复制引用
许厚义,戴群,陈桥秀..四种常见离体脱位牙贮存介质对其牙周膜成纤维细胞生物活性的影响[J].中国医学创新,2019,16(3):29-32,4.基金项目
江西省卫生计生委科技计划项目(20175294) （20175294）
