中国药业2018，Vol.27Issue(21)：23-26,4.DOI:10.3969/j.issn.1006-4931.2018.21.007
玉龙贴膏质量标准研究
Study on Quality Standard of Yulong Plaster
摘要
Abstract
Objective To establish the quality standard of Yulong Plaster. Methods Radix Paeoniae Rubra and Cortex Cinnamomi were identified by TLC mtthod. The content of peoniflorin and limit examination of aconitine were determined by HPLC method. The chromatographic column was XTerra RP18 column (250 mm × 4. 6 mm, 5 μm). The mobile phase of peoniflorin consisted of methanol-0. 05 mol/L monopotassium phosphate solution (40∶ 65), the flow rate was 1. 0 m L/min, and the detection wavelength was 230 nm. The mobile phase of aconitine consisted of methanol-0. 1％ triethylamine (70∶ 30), the flow rate was 1. 0 m L/min, and the detection wavelength was 235 nm. Results The TLC method had clear spots, strong specificity and good repeatability, the negative control had no interference. Peoniflorin showed a good linear relationship within the range of 0. 515-10. 300 μg (r = 0. 999 2), the average recovery rate was 98. 35％, and the RSD was 0. 94％ (n = 6). Aconitine showed a good linear relationship within the range of 0. 301 2-3. 012 0 μg (r = 0. 999 0), the average recovery rate was 97. 87％ and RSD was 1. 13％ (n = 6). Conclusion The established qualitative and quantitative methods are simple, reliable and accurate, which can be used for the quality control of Yulong Plaster.关键词
玉龙贴膏/质量标准/芍药苷/乌头碱/含量测定/高效液相色谱法Key words
Yulong Plaster/quality control/peoniflorin/aconitine/content determination/HPLC分类
医药卫生引用本文复制引用
刘东文,何宝凝,陈韵姿,陈淑映,李怀国..玉龙贴膏质量标准研究[J].中国药业,2018,27(21):23-26,4.基金项目
广东省佛山市科技局项目[2015AB00348] （）
