中国药业2018，Vol.27Issue(21)：35-37,3.DOI:10.3969/j.issn.1006-4931.2018.21.010
蒸发光散射检测器-高效液相色谱法测定小儿化毒胶囊中胆酸和猪去氧胆酸含量
Content Determination of Cholic Acid and Hyodeoxycholic Acid in Xiao'er Huadu Capsules by ELSD-HPLC
吴芳 1罗定强 1李青 1赵珊珊 2刘海静1
作者信息
- 1. 陕西省食品药品监督检验研究院, 陕西 西安 710061
- 2. 陕西中医药大学, 陕西 咸阳 712046
- 折叠
摘要
Abstract
Objective To establish an ELSD-HPLC method for content determination of cholic acid and hyodeoxycholic acid in Xiao'er Huadu Capsules. Methods The chromatographic column was Waters Symmetry C18 column (250 mm × 4. 6 mm, 5 μm), the mobile phase was acetonitrile-0. 1％ aceticacid (40 ∶ 60), the flow rate was 1 m L/min, the column temperature was 35 ℃, the detector was ELSD, the temperature of the drift tube was 98 ℃ and the flow rate of the nitrogen gas was 3. 0 m L/min. Results Cholic acid showed a good linear relationship within the range of 1. 068-10. 680 μg, the regression equation was Y = 1. 46 X + 5. 83 (r = 0. 999), the average recovery rate was 99. 81％ and RSD was 2. 46％ (n = 6). Hyodeoxycholic acid showed a good linear relationship within the range of 1. 121-11. 210 μg, the regression equation was Y = 1. 46 X + 5. 68 (r = 0. 999), the average recovery rate was 101. 40％ and the RSD was2. 91％ (n = 6). Conclusion The method is scientific, simple and specific, which can effectively control the quality of artificial bezoar in the preparation.关键词
小儿化毒胶囊/人工牛黄/胆酸/猪去氧胆酸/蒸发光散射检测器-高效液相色谱法/含量测定Key words
Xiao'er Huadu Capsules/artificial bezoar/cholic acid/hyodeoxycholic acid/ELSD-HPLC/content determination分类
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吴芳,罗定强,李青,赵珊珊,刘海静..蒸发光散射检测器-高效液相色谱法测定小儿化毒胶囊中胆酸和猪去氧胆酸含量[J].中国药业,2018,27(21):35-37,3.
