中国真菌学杂志2018，Vol.13Issue(6)：321-326,6.
多位点序列分型技术在热带念珠菌中的应用研究
The study of multilocus sequence typing in Candida tropicalis
王影 1项明洁 2刘锦燕 3叶书来 1周馨1
作者信息
- 1. 中国科技大学附属第一医院检验科,合肥 230001
- 2. 上海交通大学附属瑞金医院检验科,上海 200025
- 3. 上海交通大学附属瑞金医院卢湾分院放免检验科,上海 200020
- 折叠
摘要
Abstract
Objective To study the dominant genotypes of multilocus sequence typing (MLST) about Candida tropicalis in Shanghai, China.Methods The clinical Candida tropicalis isolates were collected from Ruijin Hospital, Shanghai, China, and identified using morphological analysis on CHROMagar Candida and carbohydrate assimilation tests by API 20 C AUX.Six housekeeping genes of Candida tropicalis were amplified and sequenced, and then the allelic profiles of the six gene sequences were obtained from the Candida tropicalis MLST database.The results were further analysed using the eBURST V3 package to determine the MLST clonal clusters.The phylogenetic tree was established using MEGA 6.0software.Then the drug susceptibility tests were conducted to explore the relationship between strain resistance and genotype.Results There were 47 DSTs were obtained from 92 clinical Candida tropicalis isolates, from which 32 DSTs were new to the MLST database.DST507 and DST376were the dominant genotypes, and they belong to the same clonal cluster.21 of 27clinical strains belonging to the dominant genotypes were azole-resistant strains.Conclusions MLST was a high-efficiency genotyping method for Candida tropicalis.DST507 and DST376were the dominant genotypes to Candida tropicalis.It is necessary to monitor the spread of azole-resistant Candida tropicalis, especially clonal dissemination of DST507 and DST376in the future.关键词
热带念珠菌/基因分型/MLST/DSTsKey words
Candida tropicalis/genotyping/MLST/DSTs分类
医药卫生引用本文复制引用
王影,项明洁,刘锦燕,叶书来,周馨..多位点序列分型技术在热带念珠菌中的应用研究[J].中国真菌学杂志,2018,13(6):321-326,6.
