中国真菌学杂志2018，Vol.13Issue(6)：327-333,7.
白念珠菌细胞壁蛋白Csp37抗原表位预测
Prediction of the epitopes of the cell wall protein Csp37 in Candida albicans
摘要
Abstract
Objective To predict the antigenic epitopes of the cell wall protein Csp37 in Candida albicans, and analyze their immunogenicity as vaccine targets.Methods The epitopes of Csp37 were predicted by bioinformatics method;and the basic physical and chemical properties of Csp37 protein were analyzed by ProtParam network server;signal peptide was predicted by SignalP 3.0server;transmembrane domain was predicted by TMHMM;secondary structure was predicted by GOR4;hydrophilicity, surface probability, flexible regions and antigenic index were predicted by DNAStar software.ABCPred and Syfpeithi were used to predict B and T cell epitopes, respectively.Finally, the prediction results of B and T cell epitopes were further analyzed to find the common ones.Results Prediction of B cell epitopes and T cell epitopes of the cell wall protein Csp37 of C.albicans were 9and 8respectively.The B&T cell common epitopes were 5, and the common dominant region were 45-48, 76-78, 153-158, 222-225, 303-305 aa.Conclusion Cell wall protein Csp37 of C.albicans is rich in antigenic epitopes, and has the potential to induce the cellular immune response and the humoral immune response, which could be used as a new target for vaccine against candidiasis.关键词
白念珠菌/抗原表位/生物信息学/疫苗Key words
Candida albicans/antigenic epitope/bioinformatics/vaccine分类
医药卫生引用本文复制引用
隋雪,姜远英,阎澜..白念珠菌细胞壁蛋白Csp37抗原表位预测[J].中国真菌学杂志,2018,13(6):327-333,7.基金项目
上海市自然科学基金 (17ZR1437700) （17ZR1437700）
国家自然科学基金面上项目 (81470158) （81470158）
