中国真菌学杂志2018，Vol.13Issue(6)：340-344,5.
3种方法检测艾滋病患者血和脑脊液中隐球菌的结果分析
Analysis of three methods for detecting Cryptococcus in blood and cerebrospinal fluid of AIDS patients
摘要
Abstract
Objective To compare the positive rates of Cryptococcus in blood and cerebrospinal fluid (CSF) specimens of patients with HIV/AIDS by the methods of fungal culture, ink staining and Lateral immunochromatography (LFA), hoping to provide a reference for clinicians to diagnose the HIV/AIDS combined with Cryptococcus infection.Methods Totally 1049 blood and CSF specimens of patients with HIV/AIDS in 2017 were collected for detection by the methods of fungal culture, ink staining and LFA.Besides, CSF specimens were stained with ink.SPSS software (version 14.0) was used for data analysis.Results The positive rates of blood culture, LFA, CSF culture and ink staining for 1 049 patients with HIV/AIDS were 2.8％ (19/688), 10.96％ (115/1049), 9.18％ (29/316), 17.99％ (59/328), respectively.201 patients were both tested by LFA in cerebrospinal fluid and blood at the same time, that positive rates were 27.86％ and 37.31％, respectively, with a significant difference statistically (P＜0.05).292 patients with HIV/AIDS were examined by ink staining and LFA in cerebrospinal fluid, that positive rates were 19.52％and 23.97％, respectively, with no significant difference statistically (P＞0.05).Conclusion The results of three methods showed that the positive rate of LFA was the highest.Compared with the positive rate of LFA in cerebrospinal fluid, the positive rate of blood samples was higher.So LFA is one ideal method for diagnosis of AIDS complicated with Cryptococcus infection.关键词
艾滋病/新生隐球菌/侧向免疫层析法/墨汁染色/培养Key words
HIV/AIDS/Cryptococcus neoformans/lateral immunochromatography/ink stain/culture分类
医药卫生引用本文复制引用
曹宇硕,钱雪琴,蔡金凤,陈智瑾,赵洋洋,朱召芹,卢洪洲..3种方法检测艾滋病患者血和脑脊液中隐球菌的结果分析[J].中国真菌学杂志,2018,13(6):340-344,5.基金项目
国家"十三五"重大新药创制专项 (2017ZX09304027) （2017ZX09304027）
