中国真菌学杂志2019，Vol.14Issue(2)：65-69,5.
白念珠菌ALS 3、SSA 1 基因表达在念珠菌性阴道炎免疫机制中的作用
The role of ALS3 and SSA1 gene expression of Candida albicans in the immune mechanism of vaginal candidiasis
摘要
Abstract
Objective To investigate the effect of ALS3 and SSA1 genes in Candida albicans on immune response of vaginal epithelial cells.Methods Wild strains and ALS3,SSA1 gene knockout strains(SC5314,Δals3,Δssa1)of Candida albicans were cultured and their morphology was determined.Human vaginal epithelial cell line VK2/E6E7was infected in a certain proportion.The damage of different strains to epithelial cells was evaluated by trypan blue staining and lactate dehydrogenase(LDH)activity detection.The co-culture of inflammatory cytokines and chemokines during infection was evaluated by enzyme-linked immunosorbent assay(ELISA).Results There was no significant difference in the effect of Δals3 on the germ tube length of Candida albicans,while theΔssa1 reduced the germ tube length by about 30％～40％ compared with the other two strains(P<0.001).Trypan blue staining and LDH assay showed that the cell damage ability of the three strains was proportional to the fungal load when infected with epithelial cells.Compared with wild strain,the cell damage ability ofΔssa1 was significantly reduced when infected with epithelial cells at the same MOI,and the difference was statistically significant(P<0.05),while the impact ofΔals3 mutant was weak or even slightly higher.Detection of inflammatory cytokines and chemokines showed that the ability of mutants to induce epithelial cells to produce pro-inflammatory factors and chemokines(GM-CSF,G-CSF,IL-1a,IL-8)was significantly weakened(P<0.05).Conclusion ALS3 and SSA1 gene expression played an important role in the local immune response of vaginal epithelial cells to Candida albicans infection,and SSA1 gene expression was more significant.关键词
阴道上皮细胞/白念珠菌/ALS3/SSA1/免疫Key words
vaginal epithelial cells/Candida albicans/ALS3/SSA1/immunity分类
医药卫生引用本文复制引用
高盈,王琼,梁官钊,佘晓东,史冬梅,沈永年,苏晓红,李冬梅,刘维达..白念珠菌ALS 3、SSA 1 基因表达在念珠菌性阴道炎免疫机制中的作用[J].中国真菌学杂志,2019,14(2):65-69,5.基金项目
国家重点基础研究计划(2013CB531605) （2013CB531605）
国家自然科学基金(NO.81071332) （NO.81071332）
江苏省医学科学专项计划(BL2012003) （BL2012003）
