中国真菌学杂志2019，Vol.14Issue(2)：70-77,8.
基于定量蛋白质组学的NT-89抗白念珠菌作用机制研究
Study on the mechanism of NT-89against Candida albicans based on quantitative proteomics
摘要
Abstract
Objective To analyze the differentially expressed proteins after the treatment of anti-fungal compound NT-89against Candida albicansby quantitative proteomics iTRAQ technology.Methods Total protein and cell wall proteins of C. albicans were extracted with or without NT-89treatment.The relative abundanceand differential proteins in protein extracts were detected by iTRAQ.Then the function classification of the differential proteins was performed using GO database.Results 295differential proteins were detected in extract of total protein(TP),and Ywp1p and Pga10p were most significantly down-regulated in total protein.6GPI-anchored proteins in cell wall protein(CWP)extract were found significantly downregulated. Conclusion NT-89disrupted the structural integrity and function of cell wall in C.albicans.The iTRAQ technology can provide effective reference information for drug mechanism research.关键词
NT-89/白念珠菌/细胞壁/氟化氢吡啶/GPI锚定蛋白/iTRAQKey words
NT-89/Candida albicans/cell wall/HF-pyridine/GPI-anchored protein/iTRAQ分类
医药卫生引用本文复制引用
刘昱,阎澜,姜远英..基于定量蛋白质组学的NT-89抗白念珠菌作用机制研究[J].中国真菌学杂志,2019,14(2):70-77,8.基金项目
国家自然科学基金(81573473),上海市自然科学基金(17ZR1437700) （81573473）
