中国真菌学杂志2019，Vol.14Issue(2)：90-94,5.
体外棋盘法分析粗糠柴苦素与氟康唑联合抗隐球菌作用
In vitro chessboard detection for anti-Cryptococcuseffect of rattlerin and fluconazole
摘要
Abstract
Objective To determine whether there is synergistic effect of rattlerin combined with fluconazole against Cryptococcus in vitro by the checkerboard method.Methods According to the microdilution method(M27-A3),the fluconazole and rattlerin single drug susceptibility test[FLZ,RAT]and the Fractional Inhibitory Concentration Index(FICI)were used as the judgment indicators of combined drug susceptibility test[FLZ+RAT].Results The MIC values of rattlerin to 37 strains of Cryptococcus were 32-256μg/mL.When combined with fluconazole,FICI was≤0.50,which reduced the MIC value of fluconazole by 2-16times.Conclusion Rattlerin had a certain antifungal effect on Cryptococcus,and it could synergize with fluconazole to improve the sensitivity of fluconazole to Cryptococcus.关键词
粗糠柴苦素/氟康唑/隐球菌/棋盘法/联合药敏Key words
rattlerin/fluconazole/Cryptococcus/checkerboard method/combined drug sensitivity分类
医药卫生引用本文复制引用
雷岩,方伟,张超,廖万清..体外棋盘法分析粗糠柴苦素与氟康唑联合抗隐球菌作用[J].中国真菌学杂志,2019,14(2):90-94,5.基金项目
上海市科委扬帆计划(17YF1425100) （17YF1425100）
上海市科委项目(16DZ0500401) （16DZ0500401）
上海市医学真菌分子生物学重点实验室项目(17DZ2272900)资助 （17DZ2272900）
