中国真菌学杂志2019，Vol.14Issue(2)：95-98,4.
采用分子生物学技术诊断隐球菌脑膜脑炎的研究
Study on the diagnosis of cryptococcal meningoencephalitis by molecular biological techniques
摘要
Abstract
Objective To diagnose the case of meningitis suspected of cryptococcal infection by molecular detection technique. Methods Cerebrospinal fluid samples of patients were collected,DNA was extracted,primers were designed for PCR amplification,and gene detection was performed on the amplified products by DNA chip technology.Results Sample showed positive results for Cryptococcus neoformans.Conclusion PCR amplification of primers designed from ITS conserved sequence and non-culture detection of cerebrospinal fluid samples by DNA chip technology have clinical reference value for deep fungal infections that are difficult to diagnose by traditional methods.关键词
隐球菌感染/DNA芯片/分子诊断Key words
Cryptococcus infection/DNA chip/molecular diagnosis分类
医药卫生引用本文复制引用
王光裕,陈敏,洪南,王升启,廖万清,杨英..采用分子生物学技术诊断隐球菌脑膜脑炎的研究[J].中国真菌学杂志,2019,14(2):95-98,4.基金项目
本课题受艾滋病和病毒性肝炎等重大传染病防治科技重大专项(2018ZX10101003,2018ZX10712001)、中国工程院咨询研究项目(2017-XY-35)资助. （2018ZX10101003,2018ZX10712001）
