中国肿瘤生物治疗杂志2018，Vol.25Issue(10)：1013-1020,8.DOI:10.3872/j.issn.1007-385x.2018.10.007
miR-31-5p通过调控TNS1抑制乳腺癌细胞生物学行为及放疗抵抗的分子机制
Mechanisms of miR-31-5p inhibiting biological behavior and radiotherapy resistance of breast cancer cells via regulating TNS1
余杰 1王旸 1贾彦召 1杨峥 1张森 1刘瀚文 1饶石磊 1张凯1
作者信息
- 1. 南阳市中心医院 肿瘤放疗科,河南 南阳 473009
- 折叠
摘要
Abstract
Objective: To investigate the mechanism of miR-31-5p/tension protein 1 gene (TNS1) axis modulating radiotherapy resistance in breast cancer. Methods: The breast cancer tissues and corresponding para-cancerous tissues of 21 patients with breast cancer, who underwent surgical resection at Department of Cancer Radiotherapy of Nanyang Central Hospital from July 2017 to December2017, were collected for this study; breast cancer cell lines (MCF-7, MDA-MB-23 and SKBR-3) were also collected; qPCR was applied to detect the expression level of miR-31-5p in breast cancer tissues and cell lines. The radiation resistant cell line MCF-7R was constructed by using 6 MV-X ray radiotherapy treatment. Subsequently, the influence of over-expression/kockdown of miR-31-5p on radiation sensitivity of MCF-7 and MCF-7R cells were detected by colony formation assay, Transwell assay and Annexin V-FITC/PI double staining flow cytometry assay, respectively. Moreover, luciferase reporter assay was used to verify whether TNS1 was a target gene of miR-31-5p. Results: Compared with para-cancerous tissues, normal mammary epithelial MCF-10 A cells and MCF-7 cells, miR-31-5p was low-expressed in breast cancer, cell lines and MCF-7R (all P<0.01). Over-expression of miR-31-5p resulted in inhibited invasion and promoted apoptosis of MCF-7R cells (P<0.01), whereas miR-31-5p knockdown got opposite results in MCF-7 cells. Moreover, luciferase reporter assay confirmed that TNS1 was a target gene of miR-31-5p. Over-expression of miR-31-5p inhibited invasion and increased radio-sensitivity, apoptosis of MCF-7R cell via targeting TNS1 (P<0.01), whereas knockdown of miR-31-5p significantly promoted the invasion but reduced apoptosis of MCF-7R cells (all P<0.01), and further up-regulated the radio-sensitivity of MCF-7R cells.Conclusion: miR-31-5p/TNS1 axis regulates the radiotherapy resistance of breast cancer, and over-expression of miR-31-5p may reverse the resistance of MCF-7R to radiotherapy.关键词
乳腺癌/miR-31-5p/张力蛋白1基因 (TNS1)/放疗抵抗/侵袭/凋亡Key words
breast cancer/miR-31-5p/tension protein 1 (TNS1)/radiotherapy resistance/invasion/apoptosis分类
医药卫生引用本文复制引用
余杰,王旸,贾彦召,杨峥,张森,刘瀚文,饶石磊,张凯..miR-31-5p通过调控TNS1抑制乳腺癌细胞生物学行为及放疗抵抗的分子机制[J].中国肿瘤生物治疗杂志,2018,25(10):1013-1020,8.
