中国肿瘤生物治疗杂志2018，Vol.25Issue(10)：1034-1041,8.DOI:10.3872/j.issn.1007-385x.2018.10.010
高强度聚焦超声波通过调控miR-1297/PTEN分子轴抑制胰腺癌细胞的增殖与迁移
High intensity focused ultrasound inhibits the proliferation and migration of pancreatic cancer cells via regulating miR-1297/PTEN axis
陈坚 1裴峰 2徐周敏3
作者信息
- 1. 中国人民武装警察部队上海市总队医院肿瘤科,上海 201103
- 2. 中国人民武装警察部队上海市总队医院特检科,上海 201103
- 3. 中国人民武装警察部队上海市总队医院消化科,上海 201103
- 折叠
摘要
Abstract
Objective: To explore the role and molecular mechanism of high intensity focused ultrasound (HIFU) inhibits pancreatic cancer cell proliferation and invasion via regulating miR-1297/PTEN axis. Methods: With treating the cells with HIFU for 1 to 3 seconds, the effect of HIFU on cell proliferation, invasion and apoptosis of PANC-1 cells in vitro was examined by CCK-8, Transwell and Annexin V-FITC/PI double staining flow cytometry, respectively. The effect of HIFU on expression of miR-1297 and PTEN were measured by qPCR and Western blotting. Moreover, the relationship between miR-1297 and PTEN was examined by dual luciferase report assay. Western blotting was used to detect the effect of HIFU on the expression of Akt, p-Akt (308) and p-Akt (473) after transfected with miR-1297 inhibitor and PTEN-siRNA. Results: HIFU treatment significantly inhibited the cell proliferation, invasion and promoted apoptosis of PANC-1 cells (all P<0.01), which was associated with inhibition of miR-1297 expression and activation of PTEN expression in pancreatic cancer cells (all P<0.01). Moreover, miR-1297 directly binds to the 3 ′ UTR of PTEN mRNA to suppress its expression in PANC-1 cells. Further, HIFU exposure could significantly inhibit the expression of the phosphorylation of Akt (P<0.01).Conclusion: HIFU inhibits PTEN and blocks Akt signaling by down-regulating miR-1297, thereby suppresses the occurrence and progress of pancreatic cancer.关键词
胰腺癌/高强度聚焦超声波/miR-1297/PTEN/Akt信号通路/增殖/迁移Key words
pancreatic cancer/high intensity focused ultrasound/miR-1297/PTEN/Akt signaling pathway/proliferation/migration分类
医药卫生引用本文复制引用
陈坚,裴峰,徐周敏..高强度聚焦超声波通过调控miR-1297/PTEN分子轴抑制胰腺癌细胞的增殖与迁移[J].中国肿瘤生物治疗杂志,2018,25(10):1034-1041,8.
