中国肿瘤生物治疗杂志2018，Vol.25Issue(10)：1042-1047,6.DOI:10.3872/j.issn.1007-385x.2018.10.011
LncRNA RP11-259P1.1在小细胞肺癌组织中的表达及其临床意义
Expression and clinical significance of lncRNA RP11-259P1.1 in small cell lung cancer tissues
摘要
Abstract
Objective: To explore the expression of long non-coding RNA RP11-259 P1.1 (lncRNA RP11-259 P1.1) in small cell lung cancer (SCLC) tissues and to analyze the relationship between lncRNA RP11-259 P1.1 expression and SCLC clinicopathological characteristics, as well as to investigate its effect in chemoresistance. Methods: Tissue samples, including 158 cases of tumor tissues from SCLC patients, who underwent bronchoscopic biopsy, puncture biopsy and surgical resection, 48 cases of para-cancerous tissues and 40 cases of normal lung tissues, collected from January 2012 to December 2016 in the Sixth People's Hospital of Chengdu and General Hospital of Chengdu Military Region, were used in this study. The expression of lncRNA RP11-259 P1.1 was detected by Real-time fluorescence quantitative PCR (qPCR). χ2 test was used to analyze the relationship between the expression of lncRNA RP11-259 P1.1 and the clinicopathological characteristics as well as chemotherapeutic resistance in SCLC patients. Relationship between lncRNA RP11-259 P1.1 expression and prognosis of SCLC patients was analyzed by univariate and multivariate Cox regression analysis. Results: The expression of lncRNA RP11-259 P1.1 in SCLC tissues was significantly higher than that in para-cancerous tissues and normal lung tissues (all P < 0.01). The expression of lncRNA RP11-259 P1.1 in cancer tissues of chemosensitive group was significantly lower than that of chemoresistant group (P<0.05). The expression of lncRNA RP11-259 P 1.1 was not correlated with gender and age, but significantly correlated with tumor stage, metastasis and chemosensitivity (all P<0.05). PFS and OS in patients with high lncRNA RP11-259 P1.1 expression were significantly shorter than those in patients with low expression ([12.25±1.83] vs [22.29±1.58] months, [23.55±1.35] vs [31.75±2.43] months, all P<0.01). The expression of lncRNA RP11-259 P 1.1, tumor stage and distant metastasis were the independent prognostic factors in SCLC patients (all P<0.05). Conclusion: The high expression of lncRNA RP11-259 P1.1 in SCLC tissues is associated with chemosensitivity and prognosis of SCLC patients, and may be a potential biomarker for prognosis evaluation in SCLC patients.关键词
小细胞肺癌/长链非编码RNA/RP11-259P1.1/化疗耐药/预后Key words
small cell lung carcinoma (SCLC)/long non-coding RNA/RP11-259P1.1/chemotherapy drug resistance/prognosis分类
医药卫生引用本文复制引用
李晓华,代斌,周婷,周菁,肖贞良..LncRNA RP11-259P1.1在小细胞肺癌组织中的表达及其临床意义[J].中国肿瘤生物治疗杂志,2018,25(10):1042-1047,6.基金项目
国家自然科学基金资助项目(NO.81700081) （NO.81700081）
