中国肿瘤生物治疗杂志2018，Vol.25Issue(10)：1048-1054,7.DOI:10.3872/j.issn.1007-385x.2018.10.012
Galectin-3在人乳腺癌组织中的表达及对MCF-7细胞恶性生物学行为的影响
Expression of galectin-3 in human breast cancer tissues and its effect on malignant biological behavior of MCF-7 cells
摘要
Abstract
Objective: To investigate the expression of galectin-3 protein in human breast cancer tissues and the effect of silencing galectin-3 gene on the migration, invasion and apoptosis of human breast cancer MCF-7 cells. Methods: The relative expression of galectin-3 protein in 15 cases of breast cancer tissues and corresponding para-cancerous tissues were detected by Western blotting; The expression of galectin-3 protein in paraffin sections of 100 cases of breast cancer tissues were detected by immunohistochemistry, and the correlation between galectin-3 expression and the clinicopathological characteristics of breast cancer patients was also analyzed. Galectin-3 siRNA were transfected into human breast cancer MCF-7 cells by liposome, then Real-time PCR and Western blotting were used to detect the mRNA and protein expression of galectin-3. The effect of galectin-3 gene silencing on cell migration and invasion ability of MCF-7 cells were detected by Transwell method. The effect of galectin-3 gene silencing on apoptosis of MCF-7 cells were detected by flow cytometry. Results: Western blotting detection showed that the relative expression of galectin-3 protein in breast cancer tissues were significantly higher than that in para-cancerous tissues (P<0.05); Immunohistochemistry detection showed that the positive expression rate of galectin-3 protein in breast cancer tissues was 67.00％, the positive expression rates in the lymph node metastasis, hormone receptor (ER, PR) negative groups were significantly higher (P<0.05), and the positive expression rate of galectin-3 protein were increased with the increase of TNM stage and histological grade (P<0.05); Galectin-3 siRNA transfection could significantly reduce the mRNA and protein expression of galectin-3 in MCF-7 cells (P<0.05), and reduce the invasion and migration ability but significantly improve the rate of apoptosis of MCF-7 cells (P<0.05). Conclusion: Galectin-3 is highly expressed in breast cancer tissues, and its silence can inhibit the invasion and metastasis of MCF-7 cells and induce apoptosis of MCF-7 cells. Galectin-3 can be used as a new target for biological therapy of breast cancer.关键词
乳腺癌/MCF-7细胞/半乳凝集素-3/迁移/侵袭/凋亡Key words
breast cancer/MCF-7 cell/galectin-3/migration/invasion/apoptosis分类
医药卫生引用本文复制引用
邱鹏,马立志,汤肖肖,刘晓丽,窦艳,刘月平..Galectin-3在人乳腺癌组织中的表达及对MCF-7细胞恶性生物学行为的影响[J].中国肿瘤生物治疗杂志,2018,25(10):1048-1054,7.基金项目
河北省医药卫生科技发展支持项目(No.2014JQE27031) （No.2014JQE27031）
