中国肿瘤生物治疗杂志2019，Vol.26Issue(4)：402-408,7.DOI:10.3872/j.issn.1007-385x.2019.04.006
MAGE-A1和MAGE-A3在脑胶质瘤组织中的表达及其临床意义
Expression and clinical significance of melanoma antigen genes MAGE-A1 and MAGE-A3 in glioma tissues
摘要
Abstract
Objective: To detect the expressions of melanoma antigen genes MAGE-A1 and MAGE-A3 in glioma tissues and to explore their clinical significance. Methods: Seventy-eight surgically resected glioma specimens and 15 normal brain tissue samples from donors suffered traffic accidence were collected at the Department of Neurosurgery, the Fourth Hospital of Hebei Medical University between January 2006 and January 2010, and the mRNA expressions of MAGE-A1 and MAGE-A3 in collected tissues were detected with RT-PCR; their associations with the overall survival of patients were also analyzed.The promoter methylation status of the two genes was observed with methylation specific PCR, and the relationship between the gene expressions and promoter methylation status was analyzed. The expressions of MAGE-A1 and MAGE-A3 genes in U251 and U87 glioma cell lines were detected by RT-PCR before and after the treatment with DNA methyltransferase inhibitor 5-aza-CdR and/or histone deacetylase inhibitor trichostatin A (TSA). Results:The positive expression rates of MAGE-A1 and MAGE-A3 genes in glioma tissues were 65.34％ and 38.46％, respectively; however, the two genes were not detected in 15 cases of normal brain tissues.The 5-year overall survival of patients in MAGEA1 positive expression group was shorter than that of negative expression group (P<0.05). There was significant correlation between the mRNA expressions of two genes and their promoter methylation status (all P<0.01). There was no mRNA expressions of MAGEA1 and MAGE-A3 in U87 cells untreated with 5-Aza-CdR and TSA, but a small amount of MAGE-A1 mRNA and MAGE-A3 mRNA were detected in U251 cells. TSA alone could not activate the expression of MAGE-A1 and MAGE-A3 genes. 5-Aza-CdR alone or in combination with TSA could activate the expression of both genes, and the combined effect was better than that of single administration. Conclusion: There are different degrees of MAGE-A1 and-A3 expression in glioma tissues, and the expression of MAGE-A1 is a negative prognostic factor for glioma patients. DNA promoter methylation and histone acetylation are important mechanisms of the activation of MAGE-A1 and MAGE-A3 expression.关键词
胶质瘤/U87细胞/U251细胞/黑色素瘤抗原基因-A1/黑色素瘤抗原基因-A3/DNA甲基化/组蛋白乙酰化Key words
glioma/U87 cell/U251 cell/MAGE-A1/MAGE-A3/DNA methylation/histone acetylation分类
医药卫生引用本文复制引用
国丽茹,王淑彬,桑梅香,陈丽,孟令振,单保恩..MAGE-A1和MAGE-A3在脑胶质瘤组织中的表达及其临床意义[J].中国肿瘤生物治疗杂志,2019,26(4):402-408,7.基金项目
河北省医学科学研究重点课题计划资助项目(No.20150365) （No.20150365）
