针灸临床杂志2019，Vol.35Issue(1)：50-54,5.
"通督启神"法两种电针对AD模型小鼠行为学及大脑皮层COX-2、TGF-β1表达的影响
Effects of Two Kinds of Electro-Acupuncture Therapies of Tongdu Qishen on Behavior and Expressions of COX-2 and TGF-β1 in Cerebral Cortex of AD Mice
摘要
Abstract
Objective: To observe the effect of two kinds of electro-acupuncture on behavior and expressions of inflammatory factors in cerebral cortex of the mice with Alzheimer's disease (AD) under the guidance of Tongdu Qishen theory, and to explore the mechanism and the difference of therapeutic effect of the two kinds of electroacupuncture on AD. Methods: 40 AD model mice were randomly divided into the model group, the medication group, the pulse current electro-acupuncture group and the music electro-acupuncture group; C57BL/6 mice from the same background were taken as the normal control; 10 mice in each group. The mice were bounded, the two electro-acupuncture groups were treated with needling Yintang (EX-HN3) and Baihui (GV20) for 20 minutes, and treated with needling Shuigou (GV26) without retaining the needle. The medication group was treated with Donepezil Hydrochloride (0. 92mg/kg). Besides the electro-acupuncture groups, the other three groups were given the same conditions of binding. Morris water maze was used to observe the behavioral changes of mice, and Western blot method was used to detect the expressions of inflammatory factors of the cerebral cortex in mice. Results: The escape latency of the two electro-acupuncture groups was lower than that of the model group (P ＜ 0. 01); the target quadrant swimming time/total time and the number of platform crossing in the two electro-acupuncture groups were increased than those of the model group (P ＜ 0. 05 or P ＜ 0. 01). The expressions of COX-2 and TGF-β1 of the cerebral cortex in the two electro-acupuncture groups were lower than those of the model group (P ＜ 0. 01). Conclusion: Under the guidance of Tongdu Qishen theory, the two kinds of electro-acupuncture can improve the spatial learning and memory ability of AD model mice, the mechanism may be related to inhibiting MG activation and decreasing the expressions of COX-2 and TGF-β1in cerebral cortex. There was no significant difference between the two kinds of electro-acupuncture (P＞0. 05).关键词
通督启神/阿尔茨海默病/脉冲电针/音乐电针/COX-2/TGF-β1Key words
Tongdu Qishen/Alzheimer's disease/Pulse current electro-acupuncture/Music electro-acupuncture/COX-2/TGF-β1分类
医药卫生引用本文复制引用
牟秋杰,嵇波,李昱颉,刘俊彤,杨利娟,卢梦晗,李志刚.."通督启神"法两种电针对AD模型小鼠行为学及大脑皮层COX-2、TGF-β1表达的影响[J].针灸临床杂志,2019,35(1):50-54,5.基金项目
国家自然科学基金面上项目,编号:81473774 （）
国家自然科学基金重大项目,编号:81590952. （）
