针灸临床杂志2019，Vol.35Issue(1)：58-63,封3,7.
电针对脑缺血再灌注损伤大鼠神经细胞自噬影响的实验研究
Effect of Electro-Acupuncture on Neuronal Autophagy in CIRI Rats
摘要
Abstract
Objective: To investigate the effects of electro-acupuncture on the expression of autophagy-associated protein LC3A/B in the ischemic penumbra of rats with cerebral ischemia-reperfusion injury (CIRI).Methods: 75 Sprague-Dawley rats were randomly divided into the sham operation group, the model group, the electro-acupuncutre group (EA group), the 3-MA (autophagy inhibitor) group, and EA + 3-MA group;each group was divided into three subgroups of IR24 h, IR72h and IR7d (n = 5). The composite neurological function score, the hanging line test score and the balanced walking test score were measured respectively at the three time points. The expression of autophagy-related protein LC3A/B was detected by immunohistochemistry. Results: In terms of nerve function score, the signs of neurological deficits of MCAO/R rats were alleviated in the EA group; of which there were stastistical differences between the EA group and the model group (P ＜0. 01); 3-MA group aggravated the signs of neurological deficits when compared to the model group (P ＜0. 01); however, 3-MA + EA group did not significantly improve the neurological deficit when compared to the model group (P＞ 0. 05). In terms of the LC3A/B protein expression, it showed significant increase at various time points in the model group compared to the sham operation group (P ＜ 0. 05); it reached the peak at IR72h; the expression of LC3A/B protein in the EA group was significantly higher than that in the model group at IR72h (P ＜ 0. 05); at the time of IR7 d, the expression of LC3A/B protein was significantly lower in the EA group than that in the model group (P ＜ 0. 05); the 3-MA group can significantly down-regulate the expression of LC3A/B protein; electro acupuncture intervention on the basis of the inhibitor, could up-regulate the expression of protein on IR72 h and IR7 d, there were statistical difference between the EA group and the 3-MA group (P ＜ 0. 05). Conclusion: Scalp point-to-point needling combined with electro-acupuncture can significantly improve the neurological deficit of MCAO/R rats, which has neuroprotective effects. It can regulate the expression of LC3A/B protein in the ischemic penumbra of MCAO/R rats, thereby regulating the autophagy of nerve cells. It can increase the expression of LC3A/B protein in the ischemic penumbra in the early stage of cerebral ischemia-reperfusion, moderately activate the autophagy of nerve cells in MCAO/R rats, and protect the damaged neurons; in the mid-late reperfusion period, the expression of LC3A/B protein in the ischemic penumbra was decreased, and the excessive activation of autophagy in the neurons of MCAO/R rats was inhibited, thereby antagonizing further damage of neurons.关键词
电针/脑缺血再灌注损伤/自噬/微管连接蛋白轻链3Key words
Electro-acupuncture/Cerebral ischemia reperfusion injury/Autophagy/Microtubule connexin light chain 3分类
医药卫生引用本文复制引用
孙晓伟,李洪涛,刘婷婷,孙忠人,王博,刘昊,张菶,刘丹,陈英华,金弘..电针对脑缺血再灌注损伤大鼠神经细胞自噬影响的实验研究[J].针灸临床杂志,2019,35(1):58-63,封3,7.基金项目
国家自然科学基金项目,编号:81503669,81804168 （）
黑龙江省自然基金项目,编号:H2015031 （）
黑龙江省博士后资助项目,编号:LHB-Z12248 （）
哈尔滨市科技创新人才基金项目,编号:2014RFQGJ054 （）
黑龙江中医药大学"优秀创新人才支持计划"科研项目,编号:2018RCD11. （）
