肿瘤防治研究2019，Vol.46Issue(4)：316-321,6.DOI:10.3971/j.issn.1000-8578.2019.18.1295
敲低EN2诱导肝癌细胞凋亡并提高PTEN蛋白的表达
Knockdown of EN2 Induces Apoptosis and Enhances PTEN Protein Expression in Hepatocellular Carcinoma Cells
摘要
Abstract
Objective To investigate the effect of EN2 on the apoptosis and PTEN protein expression in hepatocellular carcinoma cells. Methods Hepatoma cells HuH-7 was infected with EN2 siRNA lentivirus.qRT-PCR and Western blot methods were used to detect the interference effect. The changes of cell vitality was measured by MTT method, PI single staining method was used to determine cell cycle distribution, the apoptosis was measured by Annexin V-FITC/PI double staining, Western blot was used to measure the levels of Cleaved Caspase-3, Cleaved Caspase-9, PTEN and Cyclin B1 protein, the changes of mitochondrial membrane potential was measured by JC-1 method, and Western blot was used to measure the level of Cytochrome C protein in the cytoplasm. Results EN2 siRNA lentivirus infection could significantly reduce the expression of EN2 in hepatocellular carcinoma cells. After EN silence, the activity of hepatoma cells was decreased, the rate of apoptosis was increased, the proportion of G2/M phase in cells was increased, the levels of Cleaved Caspase-3, Cleaved Caspase-9 and PTEN protein were increased significantly, the level of Cyclin B1 protein was decreased significantly, the mitochondrial membrane potential was decreased, and the level of Cytochrome C protein in the cytoplasm was increased. Conclusion Knockdown of EN2 could block the cell cycle of liver cancer and induce the apoptosis of hepatoma cells, and the mechanism may be related to PTEN and mitochondrial apoptotic pathway.关键词
肝癌细胞/EN2/PTEN/凋亡Key words
Hepatoma cells/EN2/PTEN/Apoptosis分类
医药卫生引用本文复制引用
杨启,万春,吕新远..敲低EN2诱导肝癌细胞凋亡并提高PTEN蛋白的表达[J].肿瘤防治研究,2019,46(4):316-321,6.基金项目
国家自然科学基金-河南联合基金(U1504605) （U1504605）
