肿瘤防治研究2019，Vol.46Issue(4)：322-326,5.DOI:10.3971/j.issn.1000-8578.2019.18.1743
TAK-242对人多发性骨髓瘤细胞增殖及凋亡的影响
Effect of TAK-242 on Proliferation and Apoptosis of Human Multiple Myeloma Cells
摘要
Abstract
Objective To investigate the effect of Toll-like receptor 4 (TLR4) specific inhibitor TAK-242 on the proliferation and apoptosis of human multiple myeloma cell line RPMI8226. Methods Treated with 0, 20, 40 and 80 μmol/L TAK-242, the human multiple myeloma cell line RPMI8226 with logarithmic growth were divided into control group, group A, group B and group C, respectively. After 24 hours of culture, the cell proliferation inhibition rate was detected by CCK-8 method; the apoptosis rate was detected by Annexin-V/PI;the expression levels of TLR4 and Myd88 mRNA were detected by RT-PCR; the protein expression levels of Myd88 and NF-κB were detected by Western blot. Results Compared with the control group, the cell proliferation inhibition rate and apoptosis rate of group A, B and C were increased; the expression levels of TLR4, Myd88 mRNA and the protein expression levels of Myd88 and NF-κB were decreased; and the differences among group A, B and C were statistically significant (P<0.05). Conclusion TAK-242 could inhibit the proliferation and promote the apoptosis of human multiple myeloma cell line RPMI8226, which may be related with the inhibition of NF-κB signaling pathway function.关键词
多发性骨髓瘤/TAK-242/增殖/凋亡Key words
Multiple myeloma/TAK-242/Proliferation/Apoptosis分类
医药卫生引用本文复制引用
段萍萍,艾丽梅..TAK-242对人多发性骨髓瘤细胞增殖及凋亡的影响[J].肿瘤防治研究,2019,46(4):322-326,5.基金项目
辽宁省自然科学基金指导计划(20180550001) （20180550001）
