质谱学报2018，Vol.39Issue(5)：599-606,8.DOI:10.7538/zpxb.2018.0044
基于LTQ-Orbitrap高分辨质谱技术的柘木化学成分分析
Analysis on Chemical Constituents fromCudrania tricuspidata Bur by LTQ-Orbitrap MS
摘要
Abstract
Cudrania tricuspidata (Carr.)Bur is a traditional Chinese medicine used in the treatment of hepatitis,rheumatism,jaundice,acne,dysmenorrhea,and chronic gastritis.The chemical components of Cudrania tricuspidata (Carr.)Bur were obtained using the traditional method of extraction and separation.In this study,singlestage mass analysis that supplied molecular weight information was developed by a linear ion trap quadrupole-Orbitrap-mass spectrometry(LTQ-Orbitrap MS)and structural information was provided by multi-stage mass analysis.LC/MS technology was used to rapidly identify the chemical components of Cudrania tricuspidata (Carr.)Bur.The chromatographic separation was performed on a C18column (4.6 mm×150 mm× 5μm).Gradient elution of water(containing 0.1％formic acid)- acetonitrile was used as the mobile phase.ESI-MS analysis was operated in the range of m/z100-1 000at negativeion mode.Based on the accuracy of the relative molecular mass,fragment ions, retention behavior compared to the database,a total of 45components were identified, including 28flavonoids,11xanthones and 6other components,while 2compounds were firstly found in Cudrania tricuspidata (Carr.)Bur.It is an effective method to provide chemical information concerning the constituents in herbal medicines,which can be helpful for the quality control and further phytochemical studies of Cudrania tricuspidata (Carr.)Bur,but the method is impossible to determine the isomers and steric structures,and some small non-polar compounds can not be detected due to the lack of cleavable groups.关键词
柘木/化学成分/LTQ-Orbitrap/高分辨质谱/黄酮类/占吨酮Key words
Cudrania tricuspidata Bur/chemical component/LTQ-Orbitrap MS/flavonoids/xanthones分类
化学化工引用本文复制引用
张伽妹,郭晓宇,全庆华,姬瑞芳,孙倩倩,田婧鋆,谭鹏,刘永刚..基于LTQ-Orbitrap高分辨质谱技术的柘木化学成分分析[J].质谱学报,2018,39(5):599-606,8.基金项目
十病十药研发——普通型手足口病中药栓剂研发项目(Z161100001816020)资助 （Z161100001816020）
