中国现代中药2019,Vol.21Issue(1):71-75,81,6.DOI:10.13313/j.issn.1673-4890.20180813002
6-BA及尿素对拟巫山淫羊藿根芽形成的影响
Effects of 6-BA and Nitrogen on Buds Formation of Epimedium pseudowushanense
摘要
Abstract
Objective: To explore appropriate measures to promote formation of the buds of Epimedium pseudowushanense in late autumn. Methods: 6-BA, urea and their comprehensive treatments were chosen to either spray to the root and rhizome or to the leaves. Then the number of buds developed in winter and the weight of branches germinated from these buds was recorded in different periods, and the content of epimedin A, epimedin B, epimedin C and icariin in the dry leaves was detected by HPLC. Results: The comprehensive treatment of 6-BA 60 mg·L-1and urea 300 mg·L-1was the appropriate treatment to promote the formation of winter buds when sprayed to the rhizomes, the number of total buds was 44. 08% higher than that of the control group (P < 0. 05). And 6-BA 60 mg·L-1alone was also proved to have a good effect on the leaf yields the next year (22. 76% higher than that of the control group (P < 0. 05) when sprayed to leaves. These two treatments had no significant effect on the contents of epimedin C and the total such flavonoids in the leaves right after medical treatment and newly developed ones from winter buds. Conclusion: The comprehensive treatment of 6-BA 60 mg·L-1and urea 300 mg·L-1sprayed to rhizomes or 6-BA 60 mg·L-1sprayed to the leaves are the appropriate treatments to promote the formation of winter buds.关键词
拟巫山淫羊藿/6-BA/尿素/根芽形成/淫羊藿苷类成分含量Key words
Epimedium pseudowushanense/6-BA/urea/buds formation/flavonoids contents分类
医药卫生引用本文复制引用
田景,金华,申国安,郭宝林,姜志海,李砾,杨相波..6-BA及尿素对拟巫山淫羊藿根芽形成的影响[J].中国现代中药,2019,21(1):71-75,81,6.基金项目
中国医学科学院医学与健康科技创新工程经费资助(2017-I2M-3-013) (2017-I2M-3-013)
国家中药标准化项目(ZYBZH-C-GZ-10) (ZYBZH-C-GZ-10)