中国现代中药2019，Vol.21Issue(1)：90-94,5.DOI:10.13313/j.issn.1673-4890.20180625001
Box-Behnken响应面法优选地芍苣冬颗粒提取工艺
Optimization of Dishaojudong Granule Extraction Process by Box-Behnken Response Surface Method
摘要
Abstract
Objective: This paper aims to optimize the extraction process of Dishaojudong granule by using the Box-Behnken response surface method. Methods: The effects of immersion time, extraction time, extraction multiples and extraction times on the content of gastrotin, paeoniflorin were studied by single factor test. Based on the results of single factor, the effects of the study on the time of immersion, the time of extraction and the extraction multiples were studied. The effect of the three factors on the content of gastrotin, paeoniflorin and blood vessel diastolic rate in vitro was studied, the results were binomial equation fitting, by using the response surface method to predict the best water extraction process. Results: The predictive value of the binomial fitting equation was relatively low. In the best extraction technology conditions (soaking time for 1. 5 hours, ten times the amount of water, extraction of 1 hour every time, extraction of twice), the mean content of gastrotin was 0. 59 mg·g-1, the average content of paeoniflorin was 6. 35 mg·g-1, and the average blood vessel diastolic rate in vitro was 71. 03％. Conclusion: The method is stable and convenient and it can optimize the extraction process of the Dishaojudong granules accurately and rapidly.关键词
地芍苣冬颗粒/天麻素/芍药苷/离体血管舒张率/Box-Behnken响应面法/综合评分Key words
Dishaojudong Granula/gastrotin/paeoniflorin/blood vessel diastolic rate in vitro/Box-behnken response surface method/comprehensive score引用本文复制引用
李玉婷,张刚,姜林,郝梦,依孜提·帕力哈提,刘怡,张丽娟,张良,唐梅..Box-Behnken响应面法优选地芍苣冬颗粒提取工艺[J].中国现代中药,2019,21(1):90-94,5.基金项目
新疆地产中药民族药新药研发项目(2017-06-02) （2017-06-02）
