中国现代中药2019，Vol.21Issue(2)：182-187,198,7.DOI:10.13313/j.issn.1673-4890.20180612005
马齿苋-肉桂联用提取工艺及其对DSS诱导溃疡性结肠炎的作用研究
Study on Extraction Process of Portulaca oleracea Combining with Cinnamomum cassia and Its Effect on Ulcerative Colitis Induced by DSS
摘要
Abstract
Objective:To explore the optimal ratio of Portulaca oleracea combing with Cinnamomm cassia and evaluate their protecitive against DSS induced ulcerative colitis.Methods:Orthogonal experiment design L9 (34) was employed to optimize the extraction process.UC mice were induced by 3％ DSS and the DAI, H&E staining were enrolled to evaluated the protective effect of the extraction.The expression levels of IL-10 and TNF-α were also detected.Results:The best ratio of P.oleracea combing with C.cassia was 12:5.The extraction process was verified at 90 ℃ for 5 h.After administration of the extraction, the morphological structure of UC was improved and the inflammatory cells were almost disappeared, indicating the satisfied therapeutic effect.The expression level of IL-10 was greatly increased, while the pro-inflammatory cytokine TNF-α was decreased significantly.Conclusion:P.oleracea combing with C.cassia could improve the health conditions of UC mice via regulating the levels of inflammatory cytokines.And the extraction also can improve the morphological structure of UC.关键词
马齿苋/肉桂/联用/提取工艺/溃疡性结肠炎Key words
Portulaca oleracea/Cinnamomum cassia/combing with/extraction process/ulcerative colit分类
医药卫生引用本文复制引用
任浪浪,唐志书,梁艳妮,刘力,宋忠兴,黄文静,王征..马齿苋-肉桂联用提取工艺及其对DSS诱导溃疡性结肠炎的作用研究[J].中国现代中药,2019,21(2):182-187,198,7.基金项目
国家自然科学基金项目 (81703924) （81703924）
陕西省高校科协青年人才托举计划项目 (20160229) （20160229）
