中国组织工程研究2019，Vol.23Issue(13)：2016-2021,6.DOI:10.3969/j.issn.2095-4344.1676
人脐带间充质干细胞联合免疫干预治疗1型糖尿病小鼠的实验研究
Human umbilical cord mesenchymal stem cells combined with immunotherapy for the treatment of type 1 diabetic mice
摘要
Abstract
BACKGROUND: Type l diabetes mellitus is a T cell-mediated autoimmune disease resulting in pancreatic islet cell damage. In this study, immunotherapy was used to deal with type l diabetes mellitus and stem cell transplantation was used to repair damaged islet p cells, attempting to explore a new treatment for type l diabetes mellitus. OBJECTIVE: To observe the efficacy of human umbilical cord mesenchymal stem cells combined with immunotherapy for the treatment of type l diabetic mice. METHODS: Fifty BALB/c Foxp3-DTR-EGFP positive mice were selected, six of which were randomly selected as normal control group and the remaining of which were intraperitoneally injected with streptozotocin and diphtheria toxin to prepare an animal model of type l diabetes mellitus. After successful modeling, randomization was performed in model mice and there were four groups: model group (normal saline), immunotherapy group (subcutaneous injection of dexamethasone (10 μg) and insulin (10 μg) mixture), cell transplantation group (injection of human umbilical cord mesenchymal stem cells (1 X106 cells per mouse) through the tail vein, and combined treatment group (the combination of immunotherapy and cell transplantation as described above). At 4 weeks after treatment, changes in blood glucose, C-peptide, body mass, pancreatic histopathology and insulin-positive area were observed in each group. RESULTS AND CONCLUSION: (1) Compared with the normal control group, the blood glucose level of the model group increased (P < 0.01) the C peptide level and body mass decreased (P < 0.01), and the islet was severely atrophied, with decreased number of islet cells and reduced insulin-positive area. (2) Compared with the model group, the blood glucose level of the immunotherapy group decreased (P > 0.05), the C-peptide level and body mass did not change significantly (P > 0.05), the islet cells increased in number, and the insulin-positive area increased. (3) Compared with the model group, the blood glucose level of the cell transplantation group and the combined treatment group decreased (P > 0.05), the C peptide level and body mass increased (P < 0.05), the islet cells increased in number, and the insulin-positive area increased. These findings reveal that either human umbilical cord mesenchymal stem cells or immunotherapy can improve the islet function of type l diabetic mice, and the combination treatment has better outcomes.关键词
人脐带间充质干细胞/1型糖尿病/免疫干预/胰岛素/地塞米松/自身免疫疾病/BALB/c Foxp3-DTR-EGFP阳性小鼠/C肽Key words
Diabetes Mellitus, Type l/ Mesenchymal stem cell Transplantation/ Immunotherapy/ Stem Cells分类
医药卫生引用本文复制引用
郭波1,刘佳1,崔晓兰2,时瀚1,张社毅1,王嘉1,山霞1,王意忠1..人脐带间充质干细胞联合免疫干预治疗1型糖尿病小鼠的实验研究[J].中国组织工程研究,2019,23(13):2016-2021,6.基金项目
北京大学航天临床医学院科研基金资助项目(YN201806),课题名称:间充质干细胞联合免疫佐剂治疗1型糖尿病鼠的研究,项目负责人:王意忠 （YN201806）
