国际医药卫生导报2019，Vol.25Issue(12)：1870-1875,6.DOI:10.3760/cma.j.issn.1007-1245.2019.12.006
人参皂甙Compound K对卵巢癌细胞增殖与凋亡的影响及其机制探讨
Effect of ginsenoside compound K on proliferation and apoptosis of ovarian cancer cells and its mechanism
摘要
Abstract
Objective To detect the influence of ginsenoside compound K (CK) on proliferation,apoptosis of SKOV3 cells.Methods Cell viabilities was analyzed by CCK8 assays.Cell cycle and apoptosis rate were detected by flow cytometry.Western-blotting assay was used to analyze the expression of apoptosis-related proteins.Results After treatment with 5 μmol/L CK for 48 h,the proliferation of SKOV3 cells was significantly inhibited,and the growth cycle was arrested in G0/G1 phase,the total apoptosis rate was 11.06％.The western blotting results showed that the expressions of mitochondrial-mediated apoptotic signaling pathway-related proteins such as anti-apoptotic protein Bcl-2 and Bcl-xl decreased,while the expression of pro-apoptotic protein Bid increased.However,the apoptotic signaling pathway mediated by death receptor (Fas/FasL) did not change significantly.Conclusion CK can significantly inhibit the proliferation of ovarian cancer cells and induce cell apoptosis via mitochondrial-mediated apoptotic pathway.关键词
CK/卵巢癌/细胞增殖/细胞凋亡/线粒体介导Key words
Compound K/Ovarian cancer/Cell proliferation/Cell apoptosis/Mitochondrial mediation引用本文复制引用
刘金娥,张洪涛,李东燕,段燕,王晓鸣,单齐敏..人参皂甙Compound K对卵巢癌细胞增殖与凋亡的影响及其机制探讨[J].国际医药卫生导报,2019,25(12):1870-1875,6.基金项目
Development Project of Traditional Chinese Medical Sciences and Technology of Shandong Province (2015-498)山东省中医药科技发展计划项目(2015-498) （2015-498）
