电化学2019，Vol.25Issue(3)：400-408,9.DOI:10.13208/j.electrochem.181045
L-半胱氨酸和胱胺共自组装膜活体检测抗坏血酸
Cysteine and Cystamine Co-Self-Assembled Monolayers for in Vivo Detection of Ascorbic Acid
摘要
Abstract
Self-assembled monolayers (SAMs),which form highly ordered monolayers on the electrode surface through the gold-suffer bond,have attracted much attention in recent years.This stable layer not only can regulate the wettable properties of surface,but also can act as a promoter towards redox-active molecules.Here,we developed a simple and effective method to construct cysteine and cystamine co-self-assembled monolayer on gold microelectrode for in vivo detection ofascorbic acid (AA).The molar ratio at 1∶1 of mixed monolayer has been found the optimum to enhance the electron-transfer kinetics of AA oxidation at low potential (ca.0.10 V),meanwhile,it could resist the non-specific adsorption of protein at electrode surface.The application of the co-self-assembled monolayer is preliminarily demonstrated for in vivo detection and the basal level of striatum AA was determined to be 257 ± 30 μmol·L-1 (n =3).This study offers a general and effective approach for in vivo electrochemistry with high reliability and simplified procedures.关键词
L-半胱氨酸和胱胺/共自组装膜/抗坏血酸/抗吸附/活体Key words
cysteine and cystamine/co-self-assembled monolayer/ascorbic acid/anti-adsorption/in vivo分类
化学化工引用本文复制引用
张悦,冯涛涛,纪文亮,张美宁..L-半胱氨酸和胱胺共自组装膜活体检测抗坏血酸[J].电化学,2019,25(3):400-408,9.基金项目
We gratefully acknowledge the financial supports from the National Natural Science Foundation of China (Grant No.21874152) and Renmin University of China. （Grant No.21874152）
