动物营养学报2019，Vol.31Issue(6)：2765-2776,12.DOI:10.3969/j.issn.1006⁃267x.2019.06.037
蛋氨酸二肽在幼鲤饲料中的有效性研究及幼鲤蛋氨酸需求量的再评估
Effectiveness of Methionine Dipeptide in Juvenile Common Carp Diet and Methionine Requirement of Juvenile Common Carp Re?Evaluated by Methionine Dipeptide
摘要
Abstract
This experiment was aim to evaluate the effectiveness of methionine dipeptide ( Met?Met) in juven? ile common carp diet and to determine the methionine ( Met) requirement of juvenile common carp ( Cyprinus carpio) by adding different levels of Met?Met into the diet. Juvenile common carp with the body weight of (16.70±0.08) g randomly assigned to 7 groups with 6 replicates per group and 30 juvenile fish per replicate. Using pea concentrated protein as protein source, six experimental diets supplemented with 0, 1.5, 2.0, 2.5, 3.0 and 4.0 g/kg Met?Met were prepared based on a low Met diets with 4.8 g/kg Met and 4.4 g/kg cysteine ( Cys) to fed fish in 6 groups, which was named as M0, M1.5, M2, M2.5, M3 and M4 groups, respective?ly. Fish in the last group were fed a diet contained 10% fish meal and supplemented with 3.0 g/kg crystal DL?Met, which was named as FM group ( positive control group). The feeding trial lasted for 10 weeks. The results showed as follows: compared with M0 group, the final average body weight, weight gain rate ( WGR) and specific growth rate ( SGR) were significantly increased and the feed conversion ratio ( FCR) was signifi?cantly decreased in each Met?Met group ( P＜0.05). Taking WGR and FCR as assessment indexes, the Met re?quirements of juvenile common carp were 8.1 and 7.9 g/kg according to monodic quadratic regression model analysis, respectively. Taking protein retention rate ( PRR) as assessment index, the Met requirement of juven?ile common carp was 7.6 g/kg according to broken?line regression analysis. Diets supplemented with different levels of Met?Met all could significantly increase the contents of Met, Met+Cys, lysine ( Lys) , tryptophan ( Trp) , isoleucine ( Ile) , leucine ( Leu) , valine ( Val) and histidine ( His) in whole body, but there were no significant differences between each Met?Met group and FM group ( P＞0.05). Essential amino?acid retention rate in M0 group was significantly lower than that in other groups ( P＜0.05). Met retention rate showed a de?crease trend with the Met?Met supplemental level increasing, and the Met retention rate in M0, M1.5, M2 and M2.5 groups was significantly higher than that in FM group ( P＜0.05). Lys retention rate in M0 group was significantly lower than that in Met?Met groups except M1.5 group ( P＜0.05) , and there was no significant difference between each Met?Met group and FM group ( P＞0.05). Plasma high density lipoprotein cholesterol ( HDL?C)/total cholesterol ( TC) in M4 group was significantly higher than that in FM and M0 groups ( P＜0.05). Plasma total antioxidant capacity ( T?AOC) in M2 and M4 groups was significantly higher than that in M0 group ( P＜0.05). Plasma malondialdehyde ( MDA) content in M3 group was the lowest, which was sig?nificantly lower than that in other groups except M1.5 and M4 groups ( P＜0.05). In conclusion, suitable level of Met?Met added into a Met deficiency diet with 4.8 g/kg Met can effectively promote the growth and feed?ing, improve the transport and metabolism of lipid, and increase antioxidant ability of juvenile common carp. Met requirement of juvenile common carp is estimated to be 7.6 to 8.1 g/kg ( the supplemental level of Met?Met in the diet is 2.95 to 3.47 g/kg) , which account for 2.0% to 2.1% dietary protein, by regression analysis base on WGR, FCR and PRR. The Met supplied by Met?Met has the similar characteristics in digestion, ab?sorption and utilization with the Met from natural protein source. So, it is can be more accurate to evaluate the Met requirement of juvenile common carp using Met?Met.[ Chinese Journal of Animal Nutrition, 2019, 31 (6) :2765?2776]关键词
幼鲤/蛋氨酸二肽/蛋氨酸需求量/生长性能/回归模型/再评估Key words
juvenile common carp ( Cyprinus carpio) /Met⁃Met/Met requirement/growth performance/re⁃gression model/re⁃evaluation分类
农业科技引用本文复制引用
尹子煜,何菊云,梁晓芳,薛敏,郑银桦,谷旭,吴秀峰..蛋氨酸二肽在幼鲤饲料中的有效性研究及幼鲤蛋氨酸需求量的再评估[J].动物营养学报,2019,31(6):2765-2776,12.基金项目
国家重点研发计划项目( 2018YFD0900400) （ 2018YFD0900400）
国家重点研发计划项目(2016YFF0201800) （2016YFF0201800）
北京市现代农业产业技术体系( BA⁃IC08⁃2018) （ BA⁃IC08⁃2018）
中国农业科学院创新工程项目( CAAS⁃ASTIP⁃2017⁃FRI⁃08) （ CAAS⁃ASTIP⁃2017⁃FRI⁃08）
