动物营养学报2019，Vol.31Issue(6)：2777-2787,11.DOI:10.3969/j.issn.1006⁃267x.2019.06.038
硒源及硒水平对斜带石斑鱼幼鱼生长性能、免疫酶活性和全鱼及脊椎骨硒含量的影响
Effects of Selenium Sources and Selenium Levels on Growth Performance, Immune Enzyme activities and Selenium Contents of Whole Fish and Vertebrae for Juvenile Grouper ( Epinephelus coioide)
摘要
Abstract
This experiment was conducted to study the effects of selenium sources and selenium levels on growth performance, immune enzyme activities and selenium contents of whole fish and vertebrae for juvenile grouper ( Epinephelus coioide) , and to determine the optimal selenium requirement in diets and relative bio?availability. A total of 810 juvenile grouper with an initial average weight of (8.99±0.03) g were randomly as?signed into 9 groups with 3 replicates per group.Selenite sodium ( Se?S) and selenite methionine ( Se?Met) were used as selenite sources, and five selenium supplemental levels were 0, 0.3, 0.6, 0.9 and 1.2 mg/kg, respectively. Selenium level in a basal diet was 0.48 mg/kg; selenium levels in diets of Se?S groups were 0.82, 1.15, 1.32 and 1.79 mg/kg; selenium levels in diets of Se?Met groups were 0.81, 1.18, 1.51 and 1.94 mg/kg, respectively. Raising period was 10 weeks. The results showed as follows: 1) dietary selenium supplemental levels had significant effects on weight gain rate ( WGR) and specific growth rate ( SGR) of ju?venile grouper ( P＜0.05 ) , and had no significant effects on survival rate ( SR) and feed conversion ratio ( FCR) of juvenile grouper ( P＞0.05). When Se?S used as the selenium source, the diet supplemented with 1.15 mg/kg selenium had the highest WGR and SGR, and when Se?Met used as the selenium source, the diet supplemented with 1.18 mg/kg selenium had the highest WGR and SGR. 2 ) The different dietary selenium levels significantly affected the activities of serum alkaline phosphatase ( AKP) and liver total superoxide dis?mutase ( T?SOD) , glutathione peroxidase ( GSH?Px) , glutathione reductase ( GR) and the content of liver malondialdehyde ( MDA) ( P＜0.05). 3) The selenium contents in the whole fish and vertebrae were linear in?creased with the increased selenium levels, and vertebral selenium content was much lower than that of the whole fish. In conclusion, with the SGR as an evaluation index and the Se?S and Se?Met as the selenium sources, the optimal supplemental levels of selenium in juvenile grouper diets are 0.98 and 1.01 mg/kg, re?spectively. Under this experimental condition, the bioavailability of Se?Met is higher than that of Se?S, which is 112.5% of that of Se?S.[ Chinese Journal of Animal Nutrition, 2019, 31(6) :2777?2787]关键词
斜带石斑鱼/硒/生长性能/免疫酶活性/硒含量Key words
Epinephelus coioide/selenium/growth performance/immune enzyme activity/selenium content分类
农业科技引用本文复制引用
梁达智,马豪勇,杨奇慧,谭北平,董晓慧,迟淑艳,刘泓宇,章双,张海涛..硒源及硒水平对斜带石斑鱼幼鱼生长性能、免疫酶活性和全鱼及脊椎骨硒含量的影响[J].动物营养学报,2019,31(6):2777-2787,11.基金项目
国家自然科学基金项目(31772864,31802316) （31772864,31802316）
现代农业产业技术体系专项资金( CARS⁃47) （ CARS⁃47）
广东省社会发展领域科技计划项目(2013B021100017) （2013B021100017）
广东省自然科学基金面上项目(2015A030313621,2016A030313749) （2015A030313621,2016A030313749）
广东省创新强校工程项目(2015050206) （2015050206）
