动物营养学报2019，Vol.31Issue(6)：2800-2806,7.DOI:10.3969/j.issn.1006⁃267x.2019.06.040
饲料中添加微生态制剂对仿刺参生长、消化和免疫功能的影响
Effects of Dietary Probiotics on Growth, Digestion and Immune Function of Sea Cucumber ( Apostichopus japonicus Selenka)
摘要
Abstract
The effects of dietary probiotics on growth, digestion and immune function of sea cucumber ( Apos?tichopus japonicus Selenka) were studied in this experiment. A probiotics mainly containing Bacillus and Lac?tobacillus was used, and the living bacteria count of this probiotics was 1.0×109 CFU/mL. Sea cucumbers with an initial body weight of (15.36±0.19) g were fed with 5 experimental diets for 30 days, which supplemented with 0 ( control) , 10, 20, 30 and 40 mL/kg probiotics mixed for 9 to 12 h. Sea cucumbers were cultured in plastic cases ( 25 L) , and each case had 10 sea cucumbers and sea cucumbers in 3 cases fed one of five experi?mental diets. The results showed as follows: 1) all growth indices of sea cucumber of experimental groups with 10 and 20 mL/kg probiotics were significantly higher than those of control group ( P＜0.05). When the addi?tion increased to 30 and 40 mL/kg, there appeared evisceration and the evisceration rate was 13.33% and 26.67%, respectively, resulting in valuable contributions weakened. 2 ) The activities of protease lipase and amylase in digestive tract were increased initially and then decreased with the supplemental level of probiotics increasing, and the peak appeared in experimental groups with the probiotics supplemental levels of 20, 20 and 30 mL/kg, respectively. The activities of non?specific immune enzyme in coelomic fluid of sea cucumber in four experimental groups with 10, 20, 30 and 40 mL/kg probiotics were significantly increased compared with control group ( P＜0.05). The activities of total superoxide dismutase ( T?SOD) and catalase ( CAT) of sea cu?cumber were increased with the probiotics supplemental level increasing, but the activity of lysozyme ( LZM) of sea cucumber was firstly increased and then decreased, and the highest value was found when the probiotics supplemental level was 30 mL/kg. The results indicate that when the supplemental level of probiotics in diets is under 30 mL/kg, the growth, digestion and immune function of sea cucumber can be promoted effectively. [ Chinese Journal of Animal Nutrition, 2019, 31(6) :2800?2806]关键词
微生态制剂/仿刺参/生长/消化/免疫Key words
probiotics/sea cucumber ( Apostichopus japonicus Selenka) /growth/digestion/immune分类
农业科技引用本文复制引用
韩莎,胡炜,李成林,赵斌,陈相堂,刘兆存..饲料中添加微生态制剂对仿刺参生长、消化和免疫功能的影响[J].动物营养学报,2019,31(6):2800-2806,7.基金项目
泰山产业领军人才工程( LJNY201613) （ LJNY201613）
山东省现代农业产业技术体系刺参产业创新团队建设工程( SDAIT⁃22⁃01,SDAIT⁃22⁃05) （ SDAIT⁃22⁃01,SDAIT⁃22⁃05）
山东省农业重大应用技术创新项目 （）
山东省科技发展计划(2018GHY115042) （2018GHY115042）
山东省农业良种工程( 2017LZGC010) （ 2017LZGC010）
"海生·河口"渔业科技提升工程项目 （）
