动物营养学报2019，Vol.31Issue(6)：2865-2874,10.DOI:10.3969/j.issn.1006⁃267x.2019.06.047
发酵麸皮多糖对大鼠组织细胞因子含量及盲肠菌群结构的影响
Effects of Fermented Wheat Bran Polysaccharides on Tissue Cytokine Contents and Caecal Microflora Structure of Rats
摘要
Abstract
This experiment was conducted to investigate the effects of fermented wheat bran polysaccharides ( FWBPs) on tissue cytokine contents and caecal microflora structure of rats. Seventy?five healthy weaned SD rats were randomly assigned to 3 groups with 5 replicates per group and 5 rats per replicate. Rats in the 3 groups were lavaged daily with FWBPs solution at doses of 0 ( control group) , 100 ( low FWBPs group) and 200 mg/kg BW ( high FWBPs group). The experiment was carried out for 21 days. At the end of the experi?ment, one rat was selected from each replicate and slaughtered. The jejunum, liver and spleen tissues were col?lected to measure the cytokines such as interleukin ( IL)?1β, IL?2, IL?6 and tumor necrosis factor?α ( TNF?α) contents by enzyme linked immunosorbent assay ( ELISA) method, and the structure of caecal microflora was analyzed by Illumina HiSeq high through sequencing technology. The results showed as follows: 1) lavage of FWBPs at 100 mg/kg BW improved IL?1β, IL?2, IL?6 and TNF?α content in tissues of rats, and the TNF?α content in spleen tissue of rats was significantly improved compared with control group ( P＜0.05) ; compared with control group, the contents of IL?2 in liver tissue and IL?6 in spleen tissue were significantly decreased in high FWBPs group ( P＜0.05) ; the contents of TNF?α in jejunum tissue, IL?2 in liver tissue and IL?6 in spleen tissue of rats in low FWBPs group were significantly higher than those in high FWBPs group ( P＜0.05). 2) Lavage of FWBPs at 100 and 200 mg/kg BW both enhanced Shannon, Chao1 and ACE indices compared with control group ( P＞0.05). At the phylum level, lavage of FWBPs at 100 and 200 mg/kg BW both significantly improved the relative abundance of Firmicutes and significantly decreased the relative abundance of Bacte?roidetes in caecal chyme compared with control group ( P＜0.05). At the genus level, compared with control group, the relative abundance of Prevotella_9 in caecal chyme in low and high FWBPs groups was significantly decreased (P＜0.05), while the relative abundances of short?chain fatty acid producing bacteria Ruminococcus_1 in low FWBPs group and Coprococcus_1 in high FWBPs group were significantly increased ( P＜0.05). In con?clusion, lavage of low dose (100 mg/kg BW) FWBPs can increase the contents of cytokine in tissues and reg?ulate the structure of caecal microflora, which may further enhance the immunity of rats.[ Chinese Journal of Animal Nutrition, 2019, 31(6) :2865?2874]关键词
发酵麸皮多糖/大鼠/细胞因子/肠道菌群/高通量测序Key words
fermented wheat bran polysaccharides/rats/cytokine/gut microflora/high⁃throughput sequen⁃cing分类
轻工纺织引用本文复制引用
王文文,王园,郝希然,段元霄,安晓萍,齐景伟..发酵麸皮多糖对大鼠组织细胞因子含量及盲肠菌群结构的影响[J].动物营养学报,2019,31(6):2865-2874,10.基金项目
国家自然科学基金项目(31601969) （31601969）
内蒙古自治区高等学校科学研究项目(NJZY17070) （NJZY17070）
