康复学报2018，Vol.28Issue(1)：31-36,6.DOI:10.3724/SP.J.1329.2018.01031
片仔癀对大肠癌细胞Hedgehog信号通路调控的体内外研究
Pien Tze Huang Inhibites Colorectal Cancer Cell via Regulating Hedgehog Signaling Pathway in Vivo and in Vitro
摘要
Abstract
Objective: To explore the effect and underlying mechanism of Pien Tze Huang (PZH) inhibiting colorectal cancer cell via regulating Hedgehog signaling pathway in vivo and in vitro. Methods: Human colorectal cancer cells (HT-29) were treated with different concentrations (0, 250, 500, 1 000 μg/mL) of PZH. Cell viability and cell survival were calculated by MTT assay and colony formation assay. RT-PCR was used to determine the expression of PCNA, Bcl-XL, Bax, Survivin and the core genes of Hedgehog signaling pathway, including Shh, Ptch, Smo and Gli1. Mice were given intragastric administration of 234 mg/(kg·d) dose of PZH or saline daily, 5 days a week, and were taken materials on the 16th day in a nude mouse xenograft study. Tumor samples were analysed by immunohistochemstry for Ki-67, Shh, Ptch, Smo, Gli1. Results: HT-29 cells were inhibited by PZH in a dose dependent manner measured by MTT assay, colony formation assay and in vivo nude mouse xenograft study. The induction of apoptosis as along with the inhibition of cell proliferation was confirmed by down-regulating the expression of Ki-67, PCNA, Bcl-XL and Survivin, and up-regu lating Bax expression. The expression of Shh, Ptch, Smo and Gli1 was significantly suppressed by PZH treatment examined by RT-PCR and immunohistochemstry analysis. Conclusion: In vivo and in vitro study, it suggested that promotion of cancer cell apoptosis and inhibition of proliferation via suppression of Hedgehog signaling pathway might be one of the mechanisms by which PZH treats colorectal cancer.关键词
大肠癌/片仔癀/Hedgehog信号通路/HT-29细胞/增殖/凋亡Key words
colorectal cancer/Pien Tze Huang/Hedgehog signaling pathway/HT-29 cells/proliferation/apoptosis引用本文复制引用
庄群川,林明和,魏丽慧,彭军,林久茂..片仔癀对大肠癌细胞Hedgehog信号通路调控的体内外研究[J].康复学报,2018,28(1):31-36,6.基金项目
国家自然科学基金项目(81774121) （81774121）
福建省教育厅科技JK类项目(JK2013023) （JK2013023）
陈可冀中西医结合发展基金项目(CKJ2014016、CKJ2015002) （CKJ2014016、CKJ2015002）
