广西医科大学学报2019，Vol.36Issue(6)：863-868,6.DOI:10.16190/j.cnki.45-1211/r.2019.06.001
金桔精油通过抑制p38MAPK和NF-κB信号通路减轻脂多糖诱导的小鼠急性肺损伤
Kumquat essential oil inhibits lipopolysaccharide-induced acute lung injury by suppressing p38 MAPK and NF-κB signaling pathways in mice
摘要
Abstract
Objective:To investigate the effect and mechanisms of kumquat essential oil (KEO) on lipopolysaccharide (LPS)-induced acute lung injury (ALl) in mice.Methods:Seventytwo Kunming male mice were randomly divided 6 groups (n =12 each):control group,LPS group,dexamethasone (DXMS) group,low-dose KEO (KEOL) group,mediumdose KEO (KEOM) group,and high-dose KEO (KEOH)group.After 7 days of gavage administration,the mice were subjected to ALI except for the control group.The ALI model was established by intraperitoneally injection with LPS (10 mg/kg).The lung-to-body weight ratio was calculated.The pathological changes of lung tissues were observed under light microscope.The protein expressions of tumor necrosis factor (TNF)-α and connexin (Cx)-43 in lung tissues were determined by immunohistochemistry.The levels of interleukin (IL)-1β and IL-6 in serum were measured by enzyme linked immunosorbent assay (ELISA).The p38 mitogen-activated protein kinase (MAPK),nuclear factor (NF)-κB p65,IκBα,inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 expressions were detected by western blotting.Results:LPS-induced histological alterations in lung tissues were prevented by pretreatment of KEO.Compared with the LPS group,the lung-to-body weight ratio,the expression levels of TNF-α,Cx-43,COX-2 and iNOS as well as the serum concentrations of IL-6 and 1L-1β were decreased in KEOM and KEOH groups (P＜0.05).The expression of phosphorylated (p)-p38,p-p65,and p-IκBα was down-regulated in different doses of KEO groups (P ＜ 0.05),and that was even more marked in KEOH group.Conclusion:KEO exerted a protective effect on LPS-induced ALI in mice,which might be related to blocking the p38 MAPK and NF-κB signaling pathways and reducing the inflammatory response.关键词
金桔精油/脂多糖/急性肺损伤/MAPK/NF-κB通路Key words
kumquat essential oil/lipopolysaccharide/acute lung injury/MAPK/NF-κB pathway分类
医药卫生引用本文复制引用
陈昱晓,王娜,徐李玲,赵国强,檀雨钊,蒋伟哲,付书婕..金桔精油通过抑制p38MAPK和NF-κB信号通路减轻脂多糖诱导的小鼠急性肺损伤[J].广西医科大学学报,2019,36(6):863-868,6.基金项目
This study was supported by grants from National Natural Science Foundation of China (No.81260511),Guangxi First-Class Discipline (Pharmacy) Construction Project (No.GXF-CDP-PS-2018),Nanning Science and Technology SMEs Technology Innovation Fund Project (No.20183108) and Guangxi Innovation Drive Development Special Project-Science and Technology Major Project (No.GK-AA17202050). （No.81260511）
