湖南中医药大学学报2018，Vol.38Issue(9)：990-993,4.DOI:10.3969/j.issn.1674-070X.2018.09.005
右归丸及其拆方对肾阳虚大鼠血清T、LH和CORT的影响
Effects of Yougui Pill and Its Components on Serum T, LH, and CORT in Rats with Kidney Yang Deficiency
摘要
Abstract
Objective To explore the effect of Yougui Pill and its components on serum testosterone (T), luteinizing hormone(LH), and cortisol (CORT) in rats with kidney Yang deficiency. Methods A total of 50 3-month-old specific pathogen-free male Sprague -Dawley rats were randomly divided into normal control group, model group, Yin nourishing component group, Yang tonifying component group, and whole prescription group. All rats except those in the normal control group were intramuscularly injected with hydrocortisone in the hind legs to establish an animal model of kidney Yang deficiency. After completion of model establishment, the rats were treated for 14 consecutive days as follows: intragastric administration of corresponding medicines for the Yin nourishing component group, the Yang tonifying component group, and the whole prescription group; intragastric administration of an equal amount of normal saline for the normal control group and the model group. On the second day of medicine discontinuation, the rats were anesthetized and decapitated; serum was collected by cardiac puncture, and both testes were also collected. Body weight and testis weight of the rats were recorded, and testicular index was calculated. ELISA was used to determine the levels of T, LH, and CORT. Results Compared with the normal control group, the model group had significantly reduced body weight, testicular index, and serum levels of T, LH, and CORT (P ＜0.05). The whole prescription group had significantly increased body weight, testicular index, and serum levels of T, LH, and CORT compared with the model group (P＜0.05). Compared with the Yang tonifying component group, the Yin nourishing component group had significantly greater increases in body weight and serum LH level (P＜0.05), but significantly less increases in testicular index and serum T level (P＜0.05). Neither of the above two groups showed a significant change in serum CORT level (P＞0.05). Conclusion The whole prescription of Yougui Pill has favorable effects on body weight, testicular index, and serum levels of T, LH, and CORT in rats with kidney Yang deficiency; Yin nourishing component in the prescription reveals good effects in increasing body weight and serum LH level, and Yang tonifying component reveals good effects in increasing testicular index and serum T level; neither of the above components has an obvious effect in regulating the level of serum CORT.关键词
右归丸/拆方/阴中求阳/肾阳虚/睾酮/促黄体生成素/皮质醇Key words
Yougui Pill/seperated prescription/reinforcing Yang from Yin/kidney Yang deficiency/testosterone/luteinizing hormone/cortisol分类
医药卫生引用本文复制引用
王孙亚,周兴,宾东华,韩忠,何清湖..右归丸及其拆方对肾阳虚大鼠血清T、LH和CORT的影响[J].湖南中医药大学学报,2018,38(9):990-993,4.基金项目
国家自然科学基金(81673984) （81673984）
湖南省教育厅优秀青年项目(16B200) （16B200）
湖南中医药大学研究生创新项目(1023-0002001032) （1023-0002001032）
湖南中医药大学"一方研究生科研创新项目"(YF201701). （YF201701）
