湖南中医药大学学报2018，Vol.38Issue(9)：1003-1008,6.DOI:10.3969/j.issn.1674-070X.2018.09.008
基于低共熔溶剂提取千斤拔多糖
Extraction of Polysaccharides from Moghania macrophylla Based on Deep Eutectic Solvents
摘要
Abstract
Objective To investigate the extraction of polysaccharides from Moghania macrophylla using new green deep eutectic solvents (DESs) and related extraction process and extraction efficiency. Methods The response surface method was used to optimize the extraction process for polysaccharides from Moghania macrophylla, and the water extraction and alcohol precipitation method was used to evaluate the extraction efficiency of DES. Results The DESs composed of choline chloride and 1,3-butanediol was most suitable for the extraction of polysaccharides from Moghania macrophylla. After optimization with the response surface method, an extraction temperature of 70 °C, a water ratio of 11％, an extraction time of 41 minutes, and a liquidsolid ratio of 11 ml/g achieved a yield of polysaccharides from Moghania macrophylla of 2.47％, which was significantly higher than the yield obtained by water extraction and alcohol precipitation (P＜0.05). Conclusion DESs has the advantages of high efficiency and being environment -friendly as an extraction medium and can be used for the extraction of polysaccharides from Moghania macrophylla. Meanwhile, the results of this study provides new ideas for the use of DESs in the extraction of active components of traditional Chinese medicine.关键词
低共熔溶剂/响应面法/千斤拔/多糖Key words
deep eutectic solvent/response surface method/Moghania macrophylla/polysaccharide分类
医药卫生引用本文复制引用
熊苏慧,夏伯候,雷思敏,李诗卉,谢明霞,李亚梅,林艳..基于低共熔溶剂提取千斤拔多糖[J].湖南中医药大学学报,2018,38(9):1003-1008,6.基金项目
国家发改委标准化项目(ZYBZH-C-HUN-21) （ZYBZH-C-HUN-21）
湖南省重大科技专项(2015SK1001) （2015SK1001）
湖南省教育厅科研优秀青年项目(15B170) （15B170）
湖南省教育厅科技项目(16C1220). （16C1220）
