湖南中医药大学学报2018，Vol.38Issue(9)：1009-1011,3.DOI:10.3969/j.issn.1674-070X.2018.09.009
基于酪氨酸酶抑制活性的八白散组方研究
Prescription Formulation of Babai Powder Based on Tyrosinase Inhibitory Activity
摘要
Abstract
Objective To optimize the prescription formulation and compatibility of Babai powder with tyrosinase inhibitory activity, and to improve the whitening effect of this prescription. Methods Ultraviolet spectrophotometry was used to measure the inhibition rate of tyrosinase by water extract of Babai powder. With the inhibition rate of tyrosinase as the assessment index, uniform design was used to investigate the correlation between the dosage ratio of Babai powder and tyrosinase inhibition rate. Results In the uniform design experiment, when m (Radix Angelicae Dahuricae):m (Rhizoma Bletillae):m (Rhizoma Typhonii):m (white Poria cocos): m (Pharbitis nil) was 42:21:2:4:51, the inhibition rate of tyrosinase activity was 86％. The multivariate linear regression analysis showed that Radix Angelicae Dahuricae and white Poria cocos were the main herbal medicines for tyrosinase inhibition. The ratios of Pharbitis nil and Rhizoma Typhonii were negatively correlated with tyrosinase inhibitory activity, and their inhibitory activity tended to decrease with the increase in their ratios. Rhizoma Bletillae had no significant inhibitory effect on tyrosinase. Conclusion There is a significant difference in tyrosinase inhibitory activity between the optimized prescription formulation and compatibility of Babai powder and each single medicine, which provides a reference for further research on the prescription formulation and compatibility of Babai powder.关键词
酪氨酸酶/均匀设计/八白散/紫外分光光度法Key words
tyrosinase/uniform design/Babai powder/ultraviolet spectrophotometry分类
医药卫生引用本文复制引用
何微,王建芬,杜玉菲,刘鹰,马迎..基于酪氨酸酶抑制活性的八白散组方研究[J].湖南中医药大学学报,2018,38(9):1009-1011,3.基金项目
2014年湖南省校企合作人才培养示范基地项目(湘教通〔2014〕272号) （湘教通〔2014〕272号）
长沙医学院 2014年度大学生研究性学习与创新性课题(长医教〔2014〕10号). （长医教〔2014〕10号）
